Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/41390
Campo DC Valoridioma
dc.contributor.authorCasey, Theresaen_US
dc.contributor.authorCrodian, Jenniferen_US
dc.contributor.authorSuarez-Trujillo, Aridanyen_US
dc.contributor.authorErickson, Emilyen_US
dc.contributor.authorWeldon, Bethanyen_US
dc.contributor.authorCrow, Kristien_US
dc.contributor.authorCummings, Shelbyen_US
dc.contributor.authorChen, Yuluen_US
dc.contributor.authorShamay, Avien_US
dc.contributor.authorMabjeesh, Sameer J.en_US
dc.contributor.authorPlaut, Karenen_US
dc.contributor.otherSuarez-Trujillo, Aridany
dc.contributor.otherCasey, Theresa
dc.date.accessioned2018-06-27T09:28:44Z-
dc.date.available2018-06-27T09:28:44Z-
dc.date.issued2016en_US
dc.identifier.issn0363-6119en_US
dc.identifier.urihttp://hdl.handle.net/10553/41390-
dc.description.abstractCircadian clocks influence virtually all physiological processes, including lactation. Here, we investigate the role of the CLOCK gene in regulation of mammary epithelial cell growth and differentiation. Comparison of mammary morphology in late-pregnant wild-type and ClockΔ19 mice, showed that gland development was negatively impacted by genetic loss of a functional timing system. To understand whether these effects were due, in part, to loss of CLOCK function in the gland, the mouse mammary epithelial cell line, HC11, was transfected with short hairpin RNA that targeted Clock (shClock). Cells transfected with shClock expressed 70% less Clock mRNA than wild-type (WT) HC11 cultures, which resulted in significantly depressed levels of CLOCK protein (P < 0.05). HC11 lines carrying shClock had four-fold higher growth rates (P < 0.05), and the percentage of cells in G1 phase was significantly higher (90.1 ± 1.1% of shClock vs. 71.3 ± 3.6% of WT-HC11) following serum starvation. Quantitative-PCR (qPCR) analysis showed shClock had significant effects (P < 0.0001) on relative expression levels of Ccnd1, Wee1, and Tp63. qPCR analysis of the effect of shClock on Fasn and Cdh1 expression in undifferentiated cultures and cultures treated 96 h with dexamethasone, insulin, and prolactin (differentiated) found levels were reduced by twofold and threefold, respectively (P < 0.05), in shClock line relative to WT cultures. Abundance of CDH1 and TP63 proteins were significantly reduced in cultures transfected with shClock. These data support how CLOCK plays a role in regulation of epithelial cell growth and differentiation in the mammary gland.en_US
dc.languageengen_US
dc.relation.ispartofAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiologyen_US
dc.sourceAmerican Journal Of Physiology-Regulatory Integrative And Comparative Physiology[ISSN 0363-6119],v. 311 (6), p. R1125-R1134en_US
dc.subject3109 Ciencias veterinariasen_US
dc.subject3104 Producción Animalen_US
dc.subject240113 Fisiología animalen_US
dc.subject.otherCircadianen_US
dc.subject.otherCLOCKen_US
dc.subject.otherLactationen_US
dc.subject.otherMammary developmenten_US
dc.titleCLOCK regulates mammary epithelial cell growth and differentiationen_US
dc.typeinfo:eu-repo/semantics/Articlees
dc.typeArticlees
dc.identifier.doi10.1152/ajpregu.00032.2016
dc.identifier.scopus85002914740-
dc.identifier.isi000389643600016
dcterms.isPartOfAmerican Journal Of Physiology-Regulatory Integrative And Comparative Physiology
dcterms.sourceAmerican Journal Of Physiology-Regulatory Integrative And Comparative Physiology[ISSN 0363-6119],v. 311 (6), p. R1125-R1134
dc.contributor.authorscopusid35616075200
dc.contributor.authorscopusid8246278100
dc.contributor.authorscopusid57192267410
dc.contributor.authorscopusid56247050800
dc.contributor.authorscopusid57192268129
dc.contributor.authorscopusid57192268936
dc.contributor.authorscopusid57192273501
dc.contributor.authorscopusid57192277528
dc.contributor.authorscopusid7004358299
dc.contributor.authorscopusid7003854455
dc.contributor.authorscopusid6701676829
dc.description.lastpageR1134-
dc.identifier.issue6-
dc.description.firstpageR1125-
dc.relation.volume311-
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.identifier.wosWOS:000389643600016-
dc.contributor.daisngid304226
dc.contributor.daisngid3283671
dc.contributor.daisngid4674080
dc.contributor.daisngid8081476
dc.contributor.daisngid10338869
dc.contributor.daisngid15422017
dc.contributor.daisngid11682695
dc.contributor.daisngid6724812
dc.contributor.daisngid570641
dc.contributor.daisngid918347
dc.contributor.daisngid726145
dc.identifier.investigatorRIDP-4273-2016
dc.identifier.investigatorRIDNo ID
dc.contributor.wosstandardWOS:Casey, T
dc.contributor.wosstandardWOS:Crodian, J
dc.contributor.wosstandardWOS:Suarez-Trujillo, A
dc.contributor.wosstandardWOS:Erickson, E
dc.contributor.wosstandardWOS:Weldon, B
dc.contributor.wosstandardWOS:Crow, K
dc.contributor.wosstandardWOS:Cummings, S
dc.contributor.wosstandardWOS:Chen, YL
dc.contributor.wosstandardWOS:Shamay, A
dc.contributor.wosstandardWOS:Mabjeesh, SJ
dc.contributor.wosstandardWOS:Plaut, K
dc.date.coverdateDiciembre 2016
dc.identifier.ulpgces
dc.description.sjr1,462
dc.description.jcr2,982
dc.description.sjrqQ1
dc.description.jcrqQ2
dc.description.scieSCIE
item.fulltextCon texto completo-
item.grantfulltextopen-
crisitem.author.orcid0000-0002-9343-5016-
crisitem.author.fullNameSuárez Trujillo, Aridany-
Colección:Artículos
miniatura
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