Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/25087
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dc.contributor.authorLeizeaga, Ainaraen_US
dc.contributor.authorEstrany, Margaritaen_US
dc.contributor.authorForn, Ireneen_US
dc.contributor.authorSebastián Caumel, Martaen_US
dc.date.accessioned2018-01-12T03:30:11Z-
dc.date.accessioned2018-04-25T13:49:01Z-
dc.date.available2018-01-12T03:30:11Z-
dc.date.available2018-04-25T13:49:01Z-
dc.date.issued2017en_US
dc.identifier.issn1664-302Xen_US
dc.identifier.urihttp://hdl.handle.net/10553/25087-
dc.description.abstractA major challenge in microbial ecology is linking diversity and function to determine which microbes are actively contributing to processes occurring in situ. Bioorthogonal non-canonical amino acid tagging (BONCAT) is a promising technique for detecting and quantifying translationally active bacteria in the environment. This technique consists of incubating a bacterial sample with an analog of methionine and using click-chemistry to identify the cells that have incorporated the substrate. Here, we established an optimized protocol for the visualization of protein-synthesizing cells in oligotrophic waters that can be coupled with taxonomic identification using Catalyzed Reporter Deposition Fluorescent in Situ Hybridization. We also evaluated the use of this technique to track shifts in translational activity by comparing it with leucine incorporation, and used it to monitor temporal changes in both cultures and natural samples. Finally, we determined the optimal concentration and incubation time for substrate incorporation during BONCAT incubations at an oligotrophic site. Our results demonstrate that BONCAT is a fast and powerful semi-quantitative approach to explore the physiological status of marine bacteria.en_US
dc.formatapplication/pdf-
dc.languageengen_US
dc.relation.ispartofFrontiers in Microbiologyen_US
dc.rightsby-nc-nd-
dc.sourceFrontiers in Microbiology [ISSN 1664-302X], v. 8, article number 2360en_US
dc.subject251002 Oceanografía químicaen_US
dc.subject25 Ciencias de la tierra y del espacioen_US
dc.subject24 Ciencias de la vidaen_US
dc.subject2510 Oceanografíaen_US
dc.subject2414 Microbiologíaen_US
dc.subject.otherBONCATen_US
dc.subject.otherMarine bacteriaen_US
dc.subject.otherClick-chemistryen_US
dc.subject.otherMarine ecologyen_US
dc.subject.otherSingle-cell microbiologyen_US
dc.titleUsing click-chemistry for visualizing in situ changes of translational activity in planktonic marine bacteriaen_US
dc.typeinfo:eu-repo/semantics/articleen_US
dc.typeArticleen_US
dc.identifier.doi10.3389/fmicb.2017.02360en_US
dc.identifier.scopus85036551312-
dc.identifier.isi000416787700001-
dc.contributor.authorscopusid57194338029-
dc.contributor.authorscopusid57198447098-
dc.contributor.authorscopusid14055741200-
dc.contributor.authorscopusid14031974200-
dc.identifier.crisid-;-;-;--
dc.identifier.eissn1664-302X-
dc.identifier.issuearticle number 2360-
dc.relation.volume8en_US
dc.investigacionCienciasen_US
dc.project.referenceEcoRARE(CTM2014-60467-JIN); REMEI(CTM2015-70340-Rf)-
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess-
dc.source.typear-
dc.type2Artículoen_US
dc.identifier.externalWOS:000416787700001-
dc.utils.revisionen_US
dc.identifier.supplement-;-;-;--
dc.identifier.supplement-;-;-;--
dc.identifier.ulpgcen_US
dc.contributor.buulpgcBU-BASen_US
dc.description.sjr1,699
dc.description.jcr4,019
dc.description.sjrqQ1
dc.description.jcrqQ2
dc.description.scieSCIE
item.fulltextCon texto completo-
item.grantfulltextopen-
crisitem.author.fullNameSebastián Caumel, Marta-
Colección:Artículos
miniatura
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