Identificador persistente para citar o vincular este elemento: https://accedacris.ulpgc.es/handle/10553/142315
Título: Comparative Study of Two Immunisation Protocols in Goats Using Thiol-Sepharose Chromatography-Enriched Extracts from Adult Haemonchus contortus Worms
Autores/as: Conde De Felipe,Magnolia María 
Molina Ferrer, José Adrián 
Ferrer Quintana, Otilia Rosa 
Del Río Gismero, María Cristina 
Carmelo, Emma
Hernández Fernaud, J.R.
Rodríguez Guisado, Francisco 
Molina Caballero, José Manuel 
Ruiz Reyes, Antonio 
Clasificación UNESCO: 310903 Inmunología
Fecha de publicación: 2025
Proyectos: PID2022-136868OB-I00
Inmunización Con Proteínas Recombinantes Frente A Haemonchus Contortus en Pequeños Rumiantes Basados en Análisis Proteómicos de Extractos Purificados en Tiol-Sepharosa 
Publicación seriada: Vaccines 
Resumen: Background: A comparative analysis was conducted between two immunisation protocols using different amounts of protein extracts from adult Haemonchus contortus worms, purified by thiol-Sepharose chromatography (625 µg/animal vs. 200 µg/animal). These protocols involved either five or two inoculations of the immunogen, respectively. Methods: To evaluate the level of immunoprotection, animals were challenged with L3 of H. contortus two weeks after the last inoculation of the immunogen and humanely sacrificed at 8 weeks post-infection. Parasitological, biopathological, and serological parameters were monitored through the experiment. Parasite burden, abomasal-specific antibody responses, and histopathological changes were determined at the end of the trial. Results: The immunisation protocols resulted in similar reductions in cumulative faecal egg counts (60.5–64.9%) and the total worm burden (47.5–50%) compared to non-immunized (control) animals. Overall, these parasitological data showed an early recovery of the haematocrit (PCV) after challenge in the immunised groups relative to control. Similarly, levels of H. contortus-specific IgG and IgA antibodies increased in both the serum and gastric mucus of immunised groups. Conclusions: These findings represent a further step towards the potential application of this type of immunogen under field conditions, as protective responses (associated with a reduction in faecal egg output) were achieved using a simplified protocol, with lower immunogen doses and fewer inoculations required to induce immunoprotection, thereby mitigating the pathological effects of the parasite and reducing its ability to spread and infect susceptible hosts.
URI: https://accedacris.ulpgc.es/handle/10553/142315
ISSN: 2076-393X
DOI: 10.3390/vaccines13070708
Fuente: Vaccines [2076-393X] v. 13, (7), 708 (junio 2025)
Colección:Artículos
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