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Identificador persistente para citar o vincular este elemento: https://accedacris.ulpgc.es/handle/10553/138162
Campo DC Valoridioma
dc.contributor.authorAlkassar, Mouniraen_US
dc.contributor.authorSánchez Henao, Julián Andrésen_US
dc.contributor.authorReverté, Jaumeen_US
dc.contributor.authorBarreiro, Lourdesen_US
dc.contributor.authorRambla-Alegre, Mariaen_US
dc.contributor.authorLeonardo, Sandraen_US
dc.contributor.authorMandalakis, Manolisen_US
dc.contributor.authorPeristeraki, Panagiotaen_US
dc.contributor.authorDiogène, Jorgeen_US
dc.contributor.authorCampàs, Mònicaen_US
dc.date.accessioned2025-05-13T11:05:38Z-
dc.date.available2025-05-13T11:05:38Z-
dc.date.issued2023en_US
dc.identifier.issn1660-3397en_US
dc.identifier.urihttps://accedacris.ulpgc.es/handle/10553/138162-
dc.languageengen_US
dc.relation.ispartofMarine Drugsen_US
dc.sourceMarine Drugs [ISSN 1660-3397], v. 21, 432 (Julio 2023)en_US
dc.subject.otherTetrodotoxin (TTX)en_US
dc.subject.otherTTX analogueen_US
dc.subject.otherToxicity equivalency factor (TEF)en_US
dc.subject.otherCell-based assay (CBA)en_US
dc.subject.otherLiquid chromatography-tandem mass spectrometry (LC-MS/MS)en_US
dc.subject.otherPuffer fishen_US
dc.subject.otherLagocephalus sceleratusen_US
dc.titleEvaluation of Toxicity Equivalency Factors of Tetrodotoxin Analogues with a Neuro-2a Cell-Based Assay and Application to Puffer Fish from Greeceen_US
dc.typeinfo:eu-repo/semantics/articleen_US
dc.typeArticleen_US
dc.identifier.doi10.3390/md21080432en_US
dc.identifier.issue8-
dc.relation.volume21en_US
dc.investigacionCienciasen_US
dc.type2Artículoen_US
dc.description.notasTetrodotoxin (TTX) is a potent marine neurotoxin involved in poisoning cases, especially through the consumption of puffer fish. Knowledge of the toxicity equivalency factors (TEFs) of TTX analogues is crucial in monitoring programs to estimate the toxicity of samples analyzed with instrumental analysis methods. In this work, TTX analogues were isolated from the liver of a Lagocephalus sceleratus individual caught on South Crete coasts. A cell-based assay (CBA) for TTXs was optimized and applied to the establishment of the TEFs of 5,11-dideoxyTTX, 11-norTTX-6(S)- ol, 11-deoxyTTX and 5,6,11-trideoxyTTX. Results showed that all TTX analogues were less toxic than the parent TTX, their TEFs being in the range of 0.75–0.011. Then, different tissues of three Lagocephalus sceleratus individuals were analyzed with CBA and liquid chromatography-tandem mass spectrometry (LC-MS/MS). The obtained TEFs were applied to the TTX analogues’ concentrations obtained by LC-MS/MS analysis, providing an indication of the overall toxicity of the sample. Information about the TEFs of TTX analogues is valuable for food safety control, allowing the estimation of the risk of fish products to consumersen_US
dc.description.numberofpages22en_US
dc.utils.revisionSíen_US
dc.date.coverdateJulio 2023en_US
dc.identifier.ulpgcNoen_US
dc.contributor.buulpgcBU-BASen_US
dc.description.sjr0,88
dc.description.jcr4,9
dc.description.sjrqQ1
dc.description.jcrqQ1
dc.description.scieSCIE
dc.description.miaricds10,8
item.fulltextCon texto completo-
item.grantfulltextopen-
crisitem.author.deptGIR IUSA-ONE HEALTH 2 - Sanidad Animal de la Acuicultura y Especies Silvestres, Enfermedades Infecciosas y Seguridad Alimentaria-
crisitem.author.deptIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.orcid0000-0003-3413-4755-
crisitem.author.parentorgIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.fullNameSánchez Henao, Julián Andrés-
Colección:Artículos
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