Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/133331
DC FieldValueLanguage
dc.contributor.authorRodríguez de Cos, Irene-
dc.contributor.authorNúñez González, Raibel-
dc.contributor.authorGuedes Alonso, Raico Iván-
dc.contributor.authorPiaugeard, Sarah-
dc.contributor.authorTorres Padrón, María Esther-
dc.contributor.authorCastro Hernández, José Juan-
dc.contributor.authorSosa Ferrera, María Zoraida-
dc.contributor.authorSantana-Rodríguez, José Juan-
dc.date.accessioned2024-10-01T14:42:27Z-
dc.date.available2024-10-01T14:42:27Z-
dc.date.issued2024-
dc.identifier.issn2772-5820-
dc.identifier.otherScopus-
dc.identifier.urihttp://hdl.handle.net/10553/133331-
dc.description.abstractMarine pollution poses significant threats to ecosystems by contaminating habitats and degrading marine life. This involves the need to develop efficient methodologies to evaluate the compounds that affect marine organisms, such as steroid hormones. The study of the presence of these compounds in marine organisms like sea urchins is very interesting given their role as bioindicators because they feed on algae and are constantly in contact with sediments. Given the low concentrations of steroid hormones in marine environments, it is necessary to develop extraction procedures that allow these pollutants to be extracted and preconcentrated before chemical analyses. Of all the extraction methods, microwave-assisted extraction (MAE) has been used for its many advantages compared to traditional extraction techniques, such as easy sample handling or scarce organic solvents use, and for providing very selective extractions. This study presents the novel MAE optimisation for the extraction of 15 hormones, including five oestrogens, three androgens, four progestogens and three glucocorticoids from sea urchin tissues. The extracted hormones were subsequently determined by high-performance liquid chromatography tandem mass spectrometry. To the best of the authors' knowledge, this approach has not been previously developed. To perform extraction optimisation, different variables were studied following factorial experimental designs. The optimised extraction method showed very appropriate analytical parameters, with limits of detection between 0.21 and 20.4 ng·g-1 for the four families of studied steroid hormones, and recovery extractions over 60 % for most target compounds. After optimisation, the analytical methodology was applied to samples of three different sea urchins species (Arbacia lixula, Paracentrotus lividus, Sphaerechinus granularis) caught in different locations around the Gran Canaria island (Canary Islands, Spain). The results showed the great applicability of the optimised methodology and two target hormones, boldenone and prednisolone, which were quantified in different samples and locations. This indicates the potential of sea urchins as bioindicators of the health of marine ecosystems and of anthropogenic contamination.-
dc.languageeng-
dc.relationAnálisis de la Presencia de Microplásticos, Hormonas Esteroideasy Sus Productos de Transformación en Muestras de Sistemas de Depuración Naturaly Evaluación de Su Eliminación-
dc.relation.ispartofAdvances in Sample Preparation-
dc.sourceAdvances in Sample Preparation [ISSN 2772-5820], v. 12-
dc.subject251002 Oceanografía química-
dc.subject330811 Control de la contaminación del agua-
dc.subject230215 Hormonas-
dc.subject.otherSteroid hormones-
dc.subject.otherSea urchins-
dc.subject.otherBioindicator-
dc.subject.otherMicrowave-assisted extraction-
dc.subject.otherUHPLC-
dc.subject.otherMass spectrometry-
dc.titleDetermination of steroid hormones in sea urchins by microwave-assisted extraction and ultrahigh-performance liquid chromatography tandem mass spectrometry-
dc.typeArticle-
dc.identifier.doi10.1016/j.sampre.2024.100132-
dc.identifier.scopus85204995833-
dc.contributor.orcidNO DATA-
dc.contributor.orcid0000-0001-6088-3042-
dc.contributor.orcid0000-0002-9189-9589-
dc.contributor.orcidNO DATA-
dc.contributor.orcidNO DATA-
dc.contributor.orcidNO DATA-
dc.contributor.orcid0000-0003-3003-3607-
dc.contributor.orcid0000-0002-5635-7215-
dc.contributor.authorscopusid59344368400-
dc.contributor.authorscopusid57219235201-
dc.contributor.authorscopusid55361826300-
dc.contributor.authorscopusid59343917500-
dc.contributor.authorscopusid8452532700-
dc.contributor.authorscopusid58140867300-
dc.contributor.authorscopusid6602897915-
dc.contributor.authorscopusid56248783900-
dc.identifier.eissn2772-5820-
dc.relation.volume12-
dc.investigacionCiencias-
dc.type2Artículo-
dc.utils.revision-
dc.date.coverdateOctubre 2024-
dc.identifier.ulpgc-
dc.contributor.buulpgcBU-BAS-
dc.description.sjr0,598-
dc.description.sjrqQ2-
item.fulltextSin texto completo-
item.grantfulltextnone-
crisitem.project.principalinvestigatorGuedes Alonso, Raico Iván-
crisitem.author.deptGIR IUNAT: Análisis Químico Medioambiental-
crisitem.author.deptIU de Estudios Ambientales y Recursos Naturales-
crisitem.author.deptDepartamento de Química-
crisitem.author.deptGIR IUNAT: Análisis Químico Medioambiental-
crisitem.author.deptIU de Estudios Ambientales y Recursos Naturales-
crisitem.author.deptDepartamento de Química-
crisitem.author.deptGIR ECOAQUA: Biodiversidad y Conservación-
crisitem.author.deptIU de Investigación en Acuicultura Sostenible y Ec-
crisitem.author.deptDepartamento de Biología-
crisitem.author.deptGIR IUNAT: Análisis Químico Medioambiental-
crisitem.author.deptIU de Estudios Ambientales y Recursos Naturales-
crisitem.author.deptDepartamento de Química-
crisitem.author.deptGIR IUNAT: Análisis Químico Medioambiental-
crisitem.author.deptIU de Estudios Ambientales y Recursos Naturales-
crisitem.author.deptDepartamento de Química-
crisitem.author.orcid0000-0002-9189-9589-
crisitem.author.orcid0000-0001-7000-4419-
crisitem.author.orcid0000-0001-9577-5957-
crisitem.author.orcid0000-0003-3003-3607-
crisitem.author.orcid0000-0002-5635-7215-
crisitem.author.parentorgIU de Estudios Ambientales y Recursos Naturales-
crisitem.author.parentorgIU de Estudios Ambientales y Recursos Naturales-
crisitem.author.parentorgIU de Investigación en Acuicultura Sostenible y Ec-
crisitem.author.parentorgIU de Estudios Ambientales y Recursos Naturales-
crisitem.author.parentorgIU de Estudios Ambientales y Recursos Naturales-
crisitem.author.fullNameNúñez González, Raibel Zelideth-
crisitem.author.fullNameGuedes Alonso, Raico Iván-
crisitem.author.fullNameTorres Padrón, María Esther-
crisitem.author.fullNameCastro Hernández, José Juan-
crisitem.author.fullNameSosa Ferrera, María Zoraida-
crisitem.author.fullNameSantana Rodríguez, José Juan-
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