Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/129783
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dc.contributor.authorGonzález Dávila, Melchoren_US
dc.contributor.authorMaldonado, Maria T.en_US
dc.contributor.authorGonzález González, Aridaneen_US
dc.contributor.authorGuo, Jianen_US
dc.contributor.authorGonzalez Santana,Daviden_US
dc.contributor.authorMartel Quintana,Antera De Jesúsen_US
dc.contributor.authorSantana Casiano, Juana Magdalenaen_US
dc.date.accessioned2024-04-05T18:12:19Z-
dc.date.available2024-04-05T18:12:19Z-
dc.date.issued2024en_US
dc.identifier.issn0048-9697en_US
dc.identifier.otherWoS-
dc.identifier.urihttp://hdl.handle.net/10553/129783-
dc.description.abstractElucidating whether dissolved Cu uptake is kinetically or thermodynamically controlled, and the effects of speciation on Cu transport by phytoplankton will allow better modeling of the fate and impact of dissolved Cu in the ocean. To address these questions, we performed Cu physiological and physicochemical experiments using the model diatom, Phaeodactylum tricornutum, grown in natural North Atlantic seawater (0.44 nM Cu). Using competitive ligand equilibration-cathodic stripping voltammetry (CLE-CSV), we measured two organic ligand types released by P. tricornutum to bind Cu (L1 and L2) at concentrations of ~0.35 nM L1 and 1.3 nM L2. We also established the presence of two putative Cu-binding sites at the cell surface of P. tricornutum (S1 and S2) with log K differing by ~5 orders of magnitude (i.e., 12.9 vs. 8.1) and cell surface densities by 9-fold. Only the high-affinity binding sites, S1, exhibit reductase activity. Using voltammetric kinetic measurements and a theoretical kinetic model, we calculated the forward and dissociation rate constants of L1 and S1. Complementary 67Cu uptake experiments identified a high- and a low-affinity Cu uptake system in P. tricornutum, with half-saturation constant (Km) of 154 nM and 2.63 μM dissolved Cu, respectively. In the P. tricornutum genome, we identified a putative high-affinity Cu transporter (PtCTR49224) and a putative ZIP-like, low-affinity Cu transporter (PtZIP49400). PtCTR49224 has high homology to Homo sapiens hCTR1, which depending on the accessibility to extracellular reducing agents, the hCTR1 itself is involved in the reduction of Cu2+ to Cu+ before internalization. We combined these physiological and physicochemical data to calculate the rate constants for the internalization of Cu, and established that while the high-affinity Cu uptake system (S1) is borderline between a kinetically or thermodynamically controlled system, the low-affinity Cu transporters, S2, is thermodynamically-controlled. We revised the inverse relationship between the concentrations of inorganic complexes of essential metals (i.e., Ni, Fe, Co, Zn, Cd, Mn and Cu) in the mixed layer and the formation rate constant of metal transporters in phytoplankton, highlighting the link between the chemical properties of phytoplankton metal transporters and the availability and speciation of trace metals in the surface ocean.en_US
dc.languageengen_US
dc.relation.ispartofScience of the Total Environmenten_US
dc.sourceScience of the Total Environment [ISSN 0048-9697], v. 919, 170752, (Febrero 2024)en_US
dc.subject.otherCopperen_US
dc.subject.otherAdsorption kineticsen_US
dc.subject.otherCu transporten_US
dc.subject.otherComplexationen_US
dc.subject.otherDiatomsen_US
dc.subject.otherPhaeodactylum tricornutumen_US
dc.subject.otherCu uptakeen_US
dc.titleCu transport and complexation by the marine diatom Phaeodactylum tricornutum: Implications for trace metal complexation kinetics in the surface oceanen_US
dc.typeinfo:eu-repo/semantics/articleen_US
dc.typeArticleen_US
dc.rights.licenseBY-NC-
dc.identifier.doi10.1016/j.scitotenv.2024.170752en_US
dc.identifier.pmid38340864-
dc.identifier.scopus2-s2.0-85185331599-
dc.identifier.isi001186836900001-
dc.contributor.orcid#NODATA#-
dc.contributor.orcid#NODATA#-
dc.contributor.orcid#NODATA#-
dc.contributor.orcid#NODATA#-
dc.contributor.orcid#NODATA#-
dc.contributor.orcid#NODATA#-
dc.contributor.orcid#NODATA#-
dc.identifier.eissn1879-1026-
dc.relation.volume919en_US
dc.investigacionCienciasen_US
dc.type2Artículoen_US
dc.contributor.daisngid56151834-
dc.contributor.daisngid44646728-
dc.contributor.daisngid54262111-
dc.contributor.daisngid15036299-
dc.contributor.daisngid56117551-
dc.contributor.daisngid41239930-
dc.contributor.daisngid31951752-
dc.description.numberofpages19en_US
dc.utils.revisionen_US
dc.contributor.wosstandardWOS:Gonzalez-Davila, M-
dc.contributor.wosstandardWOS:Maldonado, MT-
dc.contributor.wosstandardWOS:Gonzalez, AG-
dc.contributor.wosstandardWOS:Guo, J-
dc.contributor.wosstandardWOS:Gonzalez-Santana, D-
dc.contributor.wosstandardWOS:Martel, A-
dc.contributor.wosstandardWOS:Santana-Casiano, JM-
dc.date.coverdateFebrero 2024en_US
dc.identifier.ulpgcen_US
dc.contributor.buulpgcBU-BASen_US
dc.description.sjr1,946-
dc.description.jcr9,8-
dc.description.sjrqQ1-
dc.description.jcrqQ1-
dc.description.scieSCIE-
dc.description.miaricds11,0-
item.fulltextSin texto completo-
item.grantfulltextnone-
crisitem.author.deptGIR IOCAG: Química Marina-
crisitem.author.deptIU de Oceanografía y Cambio Global-
crisitem.author.deptDepartamento de Química-
crisitem.author.deptGIR IOCAG: Química Marina-
crisitem.author.deptIU de Oceanografía y Cambio Global-
crisitem.author.deptDepartamento de Química-
crisitem.author.deptGIR IOCAG: Química Marina-
crisitem.author.deptIU de Oceanografía y Cambio Global-
crisitem.author.deptGIR IOCAG: Oceanografía Biológica y Algología Aplicada-
crisitem.author.deptIU de Oceanografía y Cambio Global-
crisitem.author.deptGIR IOCAG: Química Marina-
crisitem.author.deptIU de Oceanografía y Cambio Global-
crisitem.author.deptDepartamento de Química-
crisitem.author.orcid0000-0003-3230-8985-
crisitem.author.orcid0000-0002-5637-8841-
crisitem.author.orcid0000-0001-8726-7768-
crisitem.author.orcid0000-0002-7450-0505-
crisitem.author.orcid0000-0002-7930-7683-
crisitem.author.parentorgIU de Oceanografía y Cambio Global-
crisitem.author.parentorgIU de Oceanografía y Cambio Global-
crisitem.author.parentorgIU de Oceanografía y Cambio Global-
crisitem.author.parentorgIU de Oceanografía y Cambio Global-
crisitem.author.parentorgIU de Oceanografía y Cambio Global-
crisitem.author.fullNameGonzález Dávila, Melchor-
crisitem.author.fullNameGonzález González, Aridane-
crisitem.author.fullNameGonzález Santana, David-
crisitem.author.fullNameMartel Quintana,Antera De Jesús-
crisitem.author.fullNameSantana Casiano, Juana Magdalena-
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