Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/12712
Title: Evidence of a new intermediate compound of the chitin biogenesis found in a marine-derived fungus
Authors: Toledo-Marante, Francisco J. 
Mioso, Roberto 
Herrera Bravo de Laguna, Irma 
Torres, Néstor Vicente
UNESCO Clasification: 230690 Química de productos naturales orgánicos
Keywords: Chitosan
Uridine
Instrumental Technique
Chitin Synthesis
Chemical Screening
Issue Date: 2014
Journal: BMC Proceedings 
Conference: 5th Congress of the Brazilian Biotechnology Society (SBBIOTEC) 
Abstract: Chitin derivatives, chitosan and substituted chito-oligosaccharides from fungi present a wide spectrum of applications and they have been studied in many fields such as medicine, cosmetics, agriculture, aquaculture, and food as dietary supplements [1]. Chitin is a copolymer with N-acetyl-D-glucosamine units linked with β-(1-4)-glycosidic bonds which provide rigidity to the cell wall in chitinous fungi. There is evidence that the chitin synthesis is catalyzed by the chitin synthase (CS; EC 2.4.1.16), an enzyme that transfers β-1,4-linked anhydro-2-acetamido-2-deoxy-D-glucose (GlcNAc) from uridine diphosphate N-acetylglucosamine (UDP-GlcNAc) to the nonreducing end of growing chitin chains [2]. However, chitin is synthesized by the regulation of distinct isoenzymes whose number ranges in some hyphomycetes [3]. Nevertheless, there is relatively little information on the genes responsible for chitin biosynthesis in filamentous fungi, analyses of DNA fragments from taxonomically diverse fungal species have shown that most fungi have three to six chitin synthase genes [4]. Therefore, this diversity of chitin synthases makes difficult to find a unique model of regulation of the chitin pathway. The chemical screening of the biomass of a new marine-derived strain of Penicilliumroqueforti, produced by liquid-state fermentation, led to the identification of several volatile and non-volatile compounds [5]. As a result of this previous study, we have isolated and characterized a new molecule. The chemical structure of the 2-deoxy-2-phosphamino-α-D-glucopyranose isolated was elucidated on the basis of 1D and 2D NMR studies as well as other instrumental techniques. In consequence of this discovery, it has been proposed a biogenetic route aiming to explain its formation as an intermediary component of the chitin biosynthesis.
URI: http://hdl.handle.net/10553/12712
ISSN: 1753-6561
DOI: 10.1186/1753-6561-8-S4-P212
Source: BMC proceedings [ISSN 1753-6561], v. 8, (supl 4), P212 (2014)
Rights: by-nc-nd
Appears in Collections:Póster de congreso
Thumbnail
Adobe PDF (129,22 kB)
Show full item record

Page view(s)

51
checked on Jun 4, 2022

Download(s)

165
checked on Jun 4, 2022

Google ScholarTM

Check

Altmetric


Share



Export metadata



Items in accedaCRIS are protected by copyright, with all rights reserved, unless otherwise indicated.