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Title: Fiber types in cetaceans’ skeletal muscle (Longissimus dorsi): immunohistochemical study
Authors: Sierra Pulpillo, Eva María 
Arbelo Hernández, Manuel Antonio 
Caballero Cansino, María José 
Herráez Thomas, Pedro Manuel 
Espinosa De Los Monteros Y Zayas, Antonio 
Fernández Rodríguez, Antonio Jesús 
UNESCO Clasification: 310907 Patología
Issue Date: 2006
Conference: 18º Reunión de la Sociedad Española de Anatomía Patológica Veterinaria (SEAPV 2006) 
Abstract: Fiber types can be differentiated by analyzing the specific myosin heavy chain (MyHC) isoforms expressed by each fiber’s phenotype. The two major skeletal muscle fiber types are type I (slow-twitch) and type II (fast-twitch). Skeletal muscle type II fibers can be further subdivided into types IIa (fast red) and IIb (fast white). In several mammalian species a IIx (IId) myosin heavy chain isoform have been also described. Fiber-type composition varies extensively between muscles and in accordance with the functional requirements of the muscle. Endurance capacity is correlated with high percentages of type I and type IIa fibers whereas sprint capacity is correlated with high percentages of type II fibers. Samples from the dorsomedial area of the Longissimus dorsi skeletal muscle were analysed in order to compare fiber type composition among different cetacean species with different dives behaviour. Samples were collected following a standard necropsy procedure and fixed in 10% buffered formalin. Two monoclonal Anti-Myosin (Skeletal-Slow and Skeletal-fast) antibodies have been used for the localization of slow (Type I fibers) and fast (Type II) MyHC by using the immunohistochemical technique. Previous studies based in the presence of intramyocellular lipids in cetacean skeletal muscles and these new results will be presented and discussed in relationship with their behaviour.
Source: 18º Reunión de la Sociedad Española de Anatomía Patológica Veterinaria (SEAPV 2006)
Appears in Collections:Póster de congreso
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