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Title: Sanitary surveillance of the marine ecosystem of the Canary islands
Authors: Colom Rivero, Ana 
Fernández Rodríguez, Antonio Jesús 
Navarro Sarmiento, Jose 
Alonso Almorox, Paula 
Grandía Guzmán, Raiden
Marrero Ponce, Lucía 
Orós Montón, Jorge Ignacio 
Melián, A.
Cabrera, M.
Suárez-Santana, Cristian M. 
Segura Göthlin, Simona Andrea 
Felipe Jiménez, Idaira Del Carmen 
Fiorito, Carla
Arbelo Hernández, Manuel Antonio 
Sierra Pulpillo, Eva 
UNESCO Clasification: 240119 Zoología marina
240106 Ecología animal
241501 Biología molecular de microorganismos
Keywords: Sanitary Surveillance
Issue Date: 2023
Publisher: Servicio de Publicaciones y Difusión Científica de la Universidad de Las Palmas de Gran Canaria (ULPGC) 
Conference: VIII International Symposium on Marine Sciences (ISMS 2022) 
Abstract: During the last ten years, the IUSA Molecular Pathology Laboratory has set up and/or optimized numerous molecular diagnostic techniques using the polymerase chain reaction (PCR) technique for the accurate detection of marine wildlife pathogens in the Canary Islands, constituting the first systematic Sanitary Surveillance of our marine ecosystem. The presence of microorganisms including bacteria (Brucella spp., Photobacterium damsela subspecies damsela, Bartonella henselae, Listeria monocytogenes and Erysipelothrix rhusiopathiae), virus (herpesvirus, morbillivirus, poxvirus, polyomavirus, Flaviviruses including the two main lineages of West Nile Virus, Sars-CoV-2 and other compatible coronaviruses and Influenza virus (N1H1, H5, H7)), and parasites (Nasitrema delphini and N. globicephalae, Crassicauda spp. and Toxoplasma gondii) were detected, quantified, and typed by different and specific PCRs (conventional, nested, quantitative, retrotranscriptase, multiplex, SYBR Green and TaqMan, etc.). Different DNA/RNA extraction techniques are also carried out; an automated robot for these extractions has recently been incorporated into the laboratory, which greatly optimizes laboratory work. 260 cetaceans from 17 different species (Balaenoptera acutorostrata, B. physalus, Delphinus delphis, Globicephala macrorhynchus, Grampus griseus, Kogia breviceps, Lagenodelphis hosei, Mesoplodon bidens, M. densirostris, M. europaeus, M. mirus, Physeter macrocephalus, Stenella coeruleoalba, S. frontalis, Steno bredanensis, Tursiops truncatus and Ziphius cavirostris) and 72 seabirds of 14 different species (Ardea cinerea, Bubulcus ibis, Bulweria bulwerii, Calonectris diomedea borealis, Charadrius alexandrinus, Ciconia ciconia, Fratercula arctica, Larus michahellis Atlantis, Morus bassanus, Nycticorax nycticorax, Oceanodroma leucorhoa, Pandion haliaetus, Puffinus puffinus canariensis, and Sterna sandvicensis) have been analyzed to date. As a result, we have described the first detection of Brucella sp. in a cetacean stranded in the Canary Islands [1], a region with no reported cases of brucellosis for this taxon, the first detection of herpesvirus infection in Gervais’ and Sowerby’s beaked whales [2], and the first molecular confirmation of a Dolphin Morbillivirus infection in a Cuvier’s beaked whale [3], among others.
ISBN: 978-84-9042-477-3
Source: Abstracts Volume VIII International Symposium on Marine Sciences, July 2022 / coordinación, María Esther Torres Padrón, p. 268-269
Appears in Collections:Póster de congreso
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