Shewanella septentrionalis sp. nov. and Shewanella holmiensis sp. nov., isolated from Baltic Sea water and sediments

Abstract

Two bacterial strains, SP1W3T and SP1S2-7 T , were isolated from samples of water and sediments collected in Vaxholm, a town located on the Stockholm archipelago in the Baltic Sea, in November 2021. The strains were identified as novel genomic species within the genus Shewanella, based upon comparative analysis of whole genome sequence data. Strain SP1W3 T (genome size, 5.20 Mbp; G+C content, 46.0 mol%), isolated from water, was determined to be most closely related to S. hafniensis ATCC- BAA 1207 T and S. baltica NCTC 10735T, with digital DNA–DNA hybridization (dDDH) values of 61.7% and 60.4 %, respectively. Strain SP1S2- 7 T (genome size, 4.26 Mbp; G+C content, 41.5 mol%), isolated from sediments, was observed to be most closely related to S. aestuarii JCM17801T , with a pairwise dDDH value of 33.8 %. Polyphasic analyses of physiological and phenotypic charac- teristics, in addition to genomic analyses, confirmed that each of these two strains represent distinct, novel species within the genus Shewanella, for which the names Shewanella septentrionalis sp. nov. (type strain SP1W3 T=CCUG 76164 T=CECT 30651 T) and Shewanella holmiensis sp. nov. (type strain SP1S2-7T =CCUG 76165T=CECT 30652T ) are proposed.