Caenogastropod mitogenomics

Abstract

In order to further resolve the phylogenetic relationships within Caenogastropoda, the complete mitochondrial (mt) genomes of Cochlostoma hidalgoi (Cyclophoroidea), Naticarius hebraeus (Naticoidea), Galeodea echinophora (Tonnoidea), and Columbella adansoni (Buccinoidea), and the partial mt genome of Erosaria spurca (Cypraeoidea) were sequenced. All newly determined mt genomes conformed to the consensus gene order of caenogastropods, except that of C. hidalgoi, which differed in the relative positions of the trnD, trnQ, trnG, trnY, and trnT genes. Phylogenetic reconstruction of the caenogastropod tree was performed using probabilistic methods and based on the deduced amino acid sequences of concatenated mt protein coding genes. The reconstructed phylogeny recovered Architaenioglossa (superfamilies Cyclophoroidea, Ampullarioidea, and Viviparoidea) as a grade. The monophyly of Sorbeoconcha (all caenogastropods but Architaenioglossa) was supported by most but not all phylogenetic analyses (excluding Vermetoidea, which has a long branch). The relative phylogenetic position of Cerithioidea with respect to Hypsogastropoda remains unresolved. The monophyly of Hypsogastropoda (without Vermetoidea) is strongly supported. Within this clade, Littorinimorpha should be considered a grade. Several superfamilies (Abyssochrysoidea, Rissooidea, Truncatelloidea, and Naticoidea) branched off successively before a siphonate clade (Stromboidea, Cypraeoidea, Tonnoidea, Neogastropoda), which is strongly supported. The relative phylogenetic position of Vermetoidea could not be determined due to long-branch attraction artifacts. The superfamily Tonnoidea was recovered within Neogastropoda, which questions the monophyly of the latter as traditionally defined. The polyphyly of Muricoidea could be tentatively resolved excluding the families Volutidae and Babyloniidae, which would imply raising them to the rank of superfamilies.