Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/119358
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dc.contributor.authorMartín Rodríguez, Alberto Jonatanen_US
dc.contributor.authorÁlvarez-Méndez, Sergio J.en_US
dc.contributor.authorOverå, Carolineen_US
dc.contributor.authorLourenco, Tania Margaridaen_US
dc.contributor.authorNorouzitallab, Parisaen_US
dc.contributor.authorBossier, Peteren_US
dc.contributor.authorMartín, Víctor S.en_US
dc.contributor.authorFernández, José J.en_US
dc.date.accessioned2022-11-24T13:49:27Z-
dc.date.available2022-11-24T13:49:27Z-
dc.date.issued2018en_US
dc.identifier.issn2235-2988en_US
dc.identifier.urihttp://hdl.handle.net/10553/119358-
dc.description.abstractVibrio campbellii is a major pathogen in aquaculture. It is a causative agent of the so-called "luminescent vibriosis," a life-threatening condition caused by bioluminescent Vibrio spp. that often involves mass mortality of farmed shrimps. The emergence of multidrug resistant Vibrio strains raises a concern and poses a challenge for the treatment of this infection in the coming years. Inhibition of bacterial cell-to-cell communication or quorum sensing (QS) has been proposed as an alternative to antibiotic therapies. Aiming to identify novel QS disruptors, the 9H-fluroen-9yl vinyl ether derivative SAM461 was found to thwart V. campbellii bioluminescence, a QS-regulated phenotype. Phenotypic and gene expression analyses revealed, however, that the mode of action of SAM461 was unrelated to QS inhibition. Further evaluation with purified Vibrio fischeri and NanoLuc luciferases revealed enzymatic inhibition at micromolar concentrations. In silico analysis by molecular docking suggested binding of SAM461 in the active site cavities of both luciferase enzymes. Subsequent in vivo testing of SAM461 with gnotobiotic Artemia franciscana nauplii demonstrated naupliar protection against V. campbellii infection at low micromolar concentrations. Taken together, these findings suggest that suppression of luciferase activity could constitute a novel paradigm in the development of alternative anti-infective chemotherapies against luminescent vibriosis, and pave the ground for the chemical synthesis and biological characterization of derivatives with promising antimicrobial prospects.en_US
dc.languageengen_US
dc.relation.ispartofFrontiers in cellular and infection microbiologyen_US
dc.sourceFrontiers in cellular and infection microbiology [ISSN 2235-2988], v. 8, 368, (Noviembre 2018)en_US
dc.subject32 Ciencias médicasen_US
dc.subject320103 Microbiología clínicaen_US
dc.subject.otherAlternative anti-infectivesen_US
dc.subject.otherArtemiaen_US
dc.subject.otherLuciferaseen_US
dc.subject.otherVibriosisen_US
dc.subject.otherVinyl etheren_US
dc.titleThe 9H-Fluoren Vinyl Ether Derivative SAM461 Inhibits Bacterial Luciferase Activity and Protects Artemia franciscana From Luminescent Vibriosisen_US
dc.typeinfo:eu-repo/semantics/articleen_US
dc.typeArticleen_US
dc.identifier.doi10.3389/fcimb.2018.00368en_US
dc.identifier.pmid30467537-
dc.identifier.scopus2-s2.0-85057127386-
dc.contributor.orcid0000-0003-2422-129X-
dc.identifier.pmcidPMC6236115-
dc.relation.volume8en_US
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.identifier.external66386706-
dc.description.numberofpages11en_US
dc.utils.revisionen_US
dc.date.coverdateNoviembre 2018en_US
dc.identifier.ulpgcNoen_US
dc.contributor.buulpgcBU-MEDen_US
dc.description.sjr1,541
dc.description.jcr3,518
dc.description.sjrqQ1
dc.description.jcrqQ2
dc.description.scieSCIE
item.grantfulltextopen-
item.fulltextCon texto completo-
crisitem.author.deptGIR Investigación Básica y Aplicada en Ciencias de la Salud-
crisitem.author.deptDepartamento de Ciencias Clínicas-
crisitem.author.orcid0000-0003-2422-129X-
crisitem.author.parentorgDepartamento de Ciencias Clínicas-
crisitem.author.fullNameMartín Rodríguez, Alberto Jonatan-
Colección:Artículos
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