Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/115449
DC FieldValueLanguage
dc.contributor.authorÁlvarez, Claudio Andrésen_US
dc.contributor.authorSantana, Paula A.en_US
dc.contributor.authorSalinas-Parra, Nicolásen_US
dc.contributor.authorBeltrán, Dinaen_US
dc.contributor.authorGuzmán, Fannyen_US
dc.contributor.authorVega, Belindaen_US
dc.contributor.authorAcosta Arbelo, Félix Antonioen_US
dc.contributor.authorMercado, Luisen_US
dc.date.accessioned2022-06-23T08:19:45Z-
dc.date.available2022-06-23T08:19:45Z-
dc.date.issued2022en_US
dc.identifier.issn2076-2615en_US
dc.identifier.urihttp://hdl.handle.net/10553/115449-
dc.description.abstractAntimicrobial peptides (AMP) play an essential role in the innate immune system, modulating the defense response. In a previous study, we demonstrated the antimicrobial activity of synthetic hepcidin (hep20) from rainbow trout (Oncorhynchus mykiss), and its protective effect in European sea bass (Dicentrarchus labrax) challenged with Vibrio anguillarum. Additionally, we described the uptake and distribution of hep20 in different tissues and leukocyte cells. Interestingly, various AMPs characterized in high vertebrates, called host defense peptides (HDPs), also possess immunomodulation activity. For that reason, the present study explores the immunomodulatory abilities of hep20 through in vitro and in vivo studies. First, a monocyte/macrophage RTS-11 cell line from rainbow trout was used to evaluate hep20 effects on pro- and anti-inflammatory cytokines in fish leukocyte cells. Next, the European sea bass juveniles were used to determine if hep20 can regulate the expression of cytokines in fish immune tissues. The results show that hep20 was uptake inner to RTS-11 cells and was able to induce the expression of IL-10, IL-1β, and TNFα at transcriptional and protein levels. Then, the European sea bass juveniles were given intraperitoneal injections of the peptide. At 1, 3, 7, 14, and 21 days post-injection (dpi), IL-10, IL -1β, and TNFα mRNA were quantified in the anterior gut, spleen, and head kidney. The hep20 was able to up-regulate cytokine gene expression in these tissues, mainly in the head kidney. Furthermore, the evaluated cytokines showed a cyclical tendency of higher to lesser expression. Finally, a bioinformatics analysis showed that the structure adopted by hep20 is similar to the γ-core domain described for cysteine-stabilized AMP, defined as immunomodulatory and antimicrobial, which could explain the ability of hep20 to regulate the cytokine expression. This study provides new insights into immunomodulatory function complementary to the previously established antimicrobial activity of hep20, suggesting a role as an HDP in teleost fish. These facts are likely to be associated with molecular functions underpinning the protective effect of fish hepcidin against pathogens.en_US
dc.languageengen_US
dc.relation.ispartofAnimalsen_US
dc.sourceAnimals [EISSN 2076-2615], v. 12 (1586)en_US
dc.subject310502 Pisciculturaen_US
dc.subject310905 Microbiologíaen_US
dc.subject.otherHepcidinen_US
dc.subject.otherHost defense peptideen_US
dc.subject.otherCytokinesen_US
dc.subject.otherDicentrarchus labraxen_US
dc.subject.otherO. mykissen_US
dc.titleImmune Modulation Ability of Hepcidin from Teleost Fishen_US
dc.typeinfo:eu-repo/semantics/articleen_US
dc.typeArticleen_US
dc.identifier.doihttps://doi.org/10.3390/ani12121586en_US
dc.investigacionCienciasen_US
dc.type2Artículoen_US
dc.utils.revisionen_US
dc.identifier.ulpgcen_US
dc.contributor.buulpgcBU-BASen_US
dc.description.sjr0,584
dc.description.jcr2,752
dc.description.sjrqQ1
dc.description.jcrqQ1
dc.description.scieSCIE
dc.description.miaricds10,5
item.fulltextCon texto completo-
item.grantfulltextopen-
crisitem.author.deptGIR ECOAQUA: Grupo de Investigación en Acuicultura-
crisitem.author.deptIU de Investigación en Acuicultura Sostenible y Ec-
crisitem.author.deptDepartamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.orcid0000-0002-1098-7529-
crisitem.author.parentorgIU de Investigación en Acuicultura Sostenible y Ec-
crisitem.author.fullNameAcosta Arbelo, Félix Antonio-
Appears in Collections:Artículos
Adobe PDF (1,61 MB)
Show simple item record

Page view(s)

41
checked on Jul 9, 2022

Download(s)

2
checked on Jul 9, 2022

Google ScholarTM

Check

Altmetric


Share



Export metadata



Items in accedaCRIS are protected by copyright, with all rights reserved, unless otherwise indicated.