Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/114135
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dc.contributor.authorPoudel, Sunilen_US
dc.contributor.authorIzquierdo López, María Soledaden_US
dc.contributor.authorCancela, Maria Leonoren_US
dc.contributor.authorGavaia, Paulo J.en_US
dc.date.accessioned2022-03-21T13:25:55Z-
dc.date.available2022-03-21T13:25:55Z-
dc.date.issued2022en_US
dc.identifier.issn2072-6643en_US
dc.identifier.otherScopus-
dc.identifier.urihttp://hdl.handle.net/10553/114135-
dc.description.abstractDoxorubicin is a widely used chemotherapeutic drug known to induce bone loss. The mechanism behind doxorubicin-mediated bone loss is unclear, but oxidative stress has been suggested as a potential cause. Antioxidants that can counteract the toxic effect of doxorubicin on the bone would be helpful for the prevention of secondary osteoporosis. We used resveratrol, a natural antioxidant, and MitoTEMPO, a mitochondria-targeted antioxidant, to counteract doxorubicin-induced bone loss and mineralization on Sparus aurata larvae. Doxorubicin supplemented Microdiets increased bone deformities, decreased mineralization, and lipid peroxidation, whereas Resveratrol and MitoTEMPO supplemented microdiets improved mineralization, decreased bone deformities, and reversed the effects of doxorubicin in vivo and in vitro, using osteoblastic VSa13 cells. Partial Least-Squares Discriminant Analysis highlighted differences between groups on the distribution of skeletal anomalies and mineralization of skeleton elements. Calcium and Phosphorus content was negatively affected in the doxorubicin supplemented group. Doxorubicin reduced the mRNA expression of antioxidant genes, including catalase, glutathione peroxidase 1, superoxide dismutase 1, and hsp90 suggesting that ROS are central for Doxorubicin-induced bone loss. The mRNA expression of antioxidant genes was significantly increased on resveratrol alone or combined treatment. The length of intestinal villi was increased in response to antioxidants and reduced on doxorubicin. Antioxidant supplements effectively prevent bone deformities and mineralization defects, increase antioxidant response and reverse doxorubicin-induced effects on bone anomalies, mineralization, and oxidative stress. A combined treatment of doxorubicin and antioxidants was beneficial in fish larvae and showed the potential for use in preventing Doxorubicin-induced bone impairment.en_US
dc.languageengen_US
dc.relation.ispartofNutrientsen_US
dc.sourceNutrients [EISSN 2072-6643], v. 14 (6), (Marzo 2022)en_US
dc.subject310502 Pisciculturaen_US
dc.subject310406 Nutriciónen_US
dc.subject.otherBone Deformitiesen_US
dc.subject.otherDoxorubicinen_US
dc.subject.otherMineralizationen_US
dc.subject.otherMitotempoen_US
dc.subject.otherOxidative Stressen_US
dc.subject.otherResveratrolen_US
dc.titleReversal of Doxorubicin-Induced Bone Loss and Mineralization by Supplementation of Resveratrol and MitoTEMPO in the Early Development of Sparus aurataen_US
dc.typeinfo:eu-repo/semantics/Articleen_US
dc.typeArticleen_US
dc.identifier.doi10.3390/nu14061154en_US
dc.identifier.scopus85126016829-
dc.contributor.orcidNO DATA-
dc.contributor.orcidNO DATA-
dc.contributor.orcidNO DATA-
dc.contributor.orcidNO DATA-
dc.contributor.authorscopusid57482286100-
dc.contributor.authorscopusid7103111891-
dc.contributor.authorscopusid7003813966-
dc.contributor.authorscopusid6507104377-
dc.identifier.eissn2072-6643-
dc.identifier.issue6-
dc.relation.volume14en_US
dc.investigacionCienciasen_US
dc.type2Artículoen_US
dc.utils.revisionen_US
dc.date.coverdateMarzo 2022en_US
dc.identifier.ulpgcen_US
dc.contributor.buulpgcBU-BASen_US
dc.description.sjr1,418
dc.description.jcr5,717
dc.description.sjrqQ1
dc.description.jcrqQ1
dc.description.scieSCIE
dc.description.miaricds10,6
item.grantfulltextopen-
item.fulltextCon texto completo-
crisitem.author.deptGIR Grupo de Investigación en Acuicultura-
crisitem.author.deptIU de Investigación en Acuicultura Sostenible y Ec-
crisitem.author.deptDepartamento de Biología-
crisitem.author.orcid0000-0003-4297-210X-
crisitem.author.parentorgIU de Investigación en Acuicultura Sostenible y Ec-
crisitem.author.fullNameIzquierdo López, María Soledad-
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