Meta-analysis approach to the effects of live prey on the growth of Octopus vulgaris paralarvae under culture conditions

Abstract

The common octopus (Octopus vulgaris, Cuvier 1797) is a promising species for aquaculture diversification, but the massive mortality during the first life stage is the main bottleneck for its commercial production. The aim of the present study was to compare the effects of different live preys (Artemia and crustacean zoeae) and/or Artemia enrichment protocols in the paralarval growth by using a meta‐analysis approach. A total of 26 independent assays were used, including data from the bibliography and from experiments carried out by our group. Three comparisons were established: (i) crustacean zoeae vs. Artemia, (ii) different crustacean zoeae species and (iii) Artemia enriched with marine lecithin (rich in polar lipids‐PL and docosahexaenoic acid‐DHA) vs. previously used Artemia enrichments. The meta‐analysis approach allowed a quantitative review of independent studies with reliable conclusions, avoiding the subjectivity inherent to classical reviews. The outputs provided statistical confirmation of the better suitability of crustacean zoeae with respect to Artemia. However, not all crustacean species showed the same results, given that the high variability on Grapsus zoeae hampered finding significant differences with respect to the control treatment (Artemia). Nutrient composition and biometry of the different types of prey are discussed as possible causes of the differences arising from the meta‐analysis. Finally, the present results suggest that marine lecithin has a beneficial effect on paralarval growth with respect to previously used enrichments, which could be related to the increase in DHA and PL in Artemia, given the essential role of these lipid components in octopus paralarval physiology.