Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/53524
DC FieldValueLanguage
dc.contributor.authorFanjul, Luisa F.en_US
dc.contributor.authorMarrero, Isabelen_US
dc.contributor.authorGonzález, Juanen_US
dc.contributor.authorQuintana, Joséen_US
dc.contributor.authorSantana, Pinoen_US
dc.contributor.authorEstévez, Franciscoen_US
dc.contributor.authorMato, Jose Maríaen_US
dc.contributor.authorRuiz de Galarreta, Carlos M.en_US
dc.contributor.otherMarrero-Arencibia, Isabel-
dc.contributor.otherMato, Jose-
dc.contributor.otherEstevez, Francisco-
dc.contributor.otherQuintana, Jose-
dc.date.accessioned2019-02-04T17:02:43Z-
dc.date.available2019-02-04T17:02:43Z-
dc.date.issued1993en_US
dc.identifier.issn0014-2956en_US
dc.identifier.urihttp://hdl.handle.net/10553/45312-
dc.description.abstractInitial biosynthetic radiolabelling experiments with cultured granulosa cells revealed the presence of an oligosaccharide-phosphatidylinositol (glycosyl-phosphatidylinositol; (Ose)(n)PtdIns) structurally related to (Ose)(n)PtdIns-lipids isolated from other cell types. Prolactin (PRL) stimulated [H-3]glucosamine-(Ose)(n)PtdIns turnover and the rapid generation of [H-3]myristoyl-diacylglycerol in cultured follicle-stimulating hormone-(FSH)-primed granulosa cells endowed with PRL receptors. In parallel experiments performed with [H-3]myo-inositol-labelled granulosa cells, treatment with PRL stimulated (Ose)(n)PtdIns hydrolysis in a similar manner, whereas no effect on phosphoinositide (PtdIns, PtdInsP and PtdInsP2) turnover could be observed. These results strongly suggest that the cleavage of (Ose)(n)PtdIns by phosphodiesterase followed by the subsequent generation of diacylglycerol and a soluble phosphoinositol-oligosaccharide (inositol-phosphoglycan; (Ose)(n)InsP) moiety could be part of the signal-transduction mechanism linking PRL receptors to their biological effects in granulosa cells. To test this hypothesis, we examined the effect of PRL and purified (Ose)(n)InsP moiety (from rat liver membranes) on granulosa cell 3beta-hydroxysteroid dehydrogenase/DELTA5-4 isomerase (3beta-HSD) enzyme activity. Results presented show that, in FSH-primed granulosa cells, PRL (40 nM) and (Ose)(n)InsP (5 muM) prevented gonadotropin-stimulated 3beta-HSD activity. Furthermore, in undifferentiated granulosa cells where PRL receptors are absent, no effect of the hormone on 3beta-HSD activity could be observed, whereas (Ose)(n)InsP (1-10 muM) inhibited enzyme activity in a dose-dependent manner.en_US
dc.languageengen_US
dc.relation.ispartofEuropean journal of biochemistry (Print)en_US
dc.sourceEuropean Journal Of Biochemistry [ISSN 0014-2956], v. 216 (3), p. 747-755en_US
dc.subject32 Ciencias médicasen_US
dc.subject2403 Bioquímicaen_US
dc.subject.otherProtein-Kinase-Cen_US
dc.subject.otherGonadotropin-Releasing Hormoneen_US
dc.subject.otherFollicle-Stimulating-Hormoneen_US
dc.subject.otherHuman Chorionic-Gonadotropinen_US
dc.subject.otherHepatic Plasma-Membranesen_US
dc.subject.otherIsolated Rat Hepatocytesen_US
dc.subject.otherMimics Insulin Actionen_US
dc.subject.otherNerve Growth-Factoren_US
dc.subject.otherPolar Head Groupen_US
dc.subject.otherOvarian Granulosaen_US
dc.titleDoes oligosaccharide‐phosphatidylinositol (glycosyl‐phosphatidylinositol) hydrolysis mediate prolactin signal transduction in granulosa cells?en_US
dc.typeinfo:eu-repo/semantics/Articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1111/j.1432-1033.1993.tb18194.xen_US
dc.identifier.scopus0027430799-
dc.identifier.isiA1993LX85200006-
dcterms.isPartOfEuropean Journal Of Biochemistry-
dcterms.sourceEuropean Journal Of Biochemistry[ISSN 0014-2956],v. 216 (3), p. 747-755-
dc.contributor.authorscopusid7004158812-
dc.contributor.authorscopusid35936487800-
dc.contributor.authorscopusid57198495832-
dc.contributor.authorscopusid8681043500-
dc.contributor.authorscopusid7003526778-
dc.contributor.authorscopusid7003810011-
dc.contributor.authorscopusid55399368600-
dc.contributor.authorscopusid7003806034-
dc.description.lastpage755en_US
dc.identifier.issue3-
dc.description.firstpage747en_US
dc.relation.volume216en_US
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.identifier.wosWOS:A1993LX85200006-
dc.contributor.daisngid1127140-
dc.contributor.daisngid1533885-
dc.contributor.daisngid3844163-
dc.contributor.daisngid7986161-
dc.contributor.daisngid136721-
dc.contributor.daisngid128315-
dc.contributor.daisngid31449715-
dc.contributor.daisngid329218-
dc.contributor.daisngid384944-
dc.contributor.daisngid26350-
dc.contributor.daisngid1664323-
dc.identifier.investigatorRIDQ-9437-2016-
dc.identifier.investigatorRIDA-5187-2011-
dc.identifier.investigatorRIDK-5125-2014-
dc.identifier.investigatorRIDK-5709-2014-
dc.description.numberofpages9en_US
dc.utils.revisionen_US
dc.contributor.wosstandardWOS:FANJUL, LF-
dc.contributor.wosstandardWOS:MARRERO, I-
dc.contributor.wosstandardWOS:GONALEZ, J-
dc.contributor.wosstandardWOS:QUINTANA, J-
dc.contributor.wosstandardWOS:SANTANA, P-
dc.contributor.wosstandardWOS:ESTEVEZ, F-
dc.contributor.wosstandardWOS:MATO, JM-
dc.contributor.wosstandardWOS:DEGALARRETA, CMR-
dc.date.coverdateSeptiembre 1993en_US
dc.identifier.ulpgcen_US
dc.contributor.buulpgcBU-MEDen_US
item.fulltextSin texto completo-
item.grantfulltextnone-
crisitem.author.deptSeñalización Intracelular y Expresión Génica-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Bioquímica y Biología Molecular, Fisiología, Genética e Inmunología.-
crisitem.author.deptDepartamento de Bioquímica y Biología Molecular, Fisiología, Genética e Inmunología.-
crisitem.author.deptBioquímica Farmacológica-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Bioquímica y Biología Molecular, Fisiología, Genética e Inmunología.-
crisitem.author.deptBioquímica Farmacológica-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Bioquímica y Biología Molecular, Fisiología, Genética e Inmunología.-
crisitem.author.orcid0000-0003-3732-9929-
crisitem.author.orcid0000-0001-8225-4538-
crisitem.author.orcid0000-0002-9728-2774-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.fullNameFanjul Rodríguez, Luisa Fernanda-
crisitem.author.fullNameMarrero Arencibia, María Isabel-
crisitem.author.fullNameQuintana Aguiar, José Martín-
crisitem.author.fullNameEstévez Rosas, Francisco Jesús-
Appears in Collections:Artículos
Show simple item record

SCOPUSTM   
Citations

8
checked on May 9, 2021

WEB OF SCIENCETM
Citations

8
checked on May 9, 2021

Page view(s)

19
checked on May 15, 2021

Google ScholarTM

Check

Altmetric


Share



Export metadata



Items in accedaCRIS are protected by copyright, with all rights reserved, unless otherwise indicated.