Please use this identifier to cite or link to this item:
http://hdl.handle.net/10553/53524
DC Field | Value | Language |
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dc.contributor.author | Fanjul Rodríguez, Luisa Fernanda | en_US |
dc.contributor.author | Marrero Arencibia, María Isabel | en_US |
dc.contributor.author | González, Juan | en_US |
dc.contributor.author | Quintana, José | en_US |
dc.contributor.author | Santana, Pino | en_US |
dc.contributor.author | Estévez Rosas, Francisco Jesús | en_US |
dc.contributor.author | Mato, Jose María | en_US |
dc.contributor.author | Ruiz de Galarreta, Carlos M. | en_US |
dc.contributor.other | Marrero-Arencibia, Isabel | - |
dc.contributor.other | Mato, Jose | - |
dc.contributor.other | Estevez, Francisco | - |
dc.contributor.other | Quintana, Jose | - |
dc.date.accessioned | 2019-02-04T17:02:43Z | - |
dc.date.available | 2019-02-04T17:02:43Z | - |
dc.date.issued | 1993 | en_US |
dc.identifier.issn | 0014-2956 | en_US |
dc.identifier.uri | http://hdl.handle.net/10553/45312 | - |
dc.description.abstract | Initial biosynthetic radiolabelling experiments with cultured granulosa cells revealed the presence of an oligosaccharide-phosphatidylinositol (glycosyl-phosphatidylinositol; (Ose)(n)PtdIns) structurally related to (Ose)(n)PtdIns-lipids isolated from other cell types. Prolactin (PRL) stimulated [H-3]glucosamine-(Ose)(n)PtdIns turnover and the rapid generation of [H-3]myristoyl-diacylglycerol in cultured follicle-stimulating hormone-(FSH)-primed granulosa cells endowed with PRL receptors. In parallel experiments performed with [H-3]myo-inositol-labelled granulosa cells, treatment with PRL stimulated (Ose)(n)PtdIns hydrolysis in a similar manner, whereas no effect on phosphoinositide (PtdIns, PtdInsP and PtdInsP2) turnover could be observed. These results strongly suggest that the cleavage of (Ose)(n)PtdIns by phosphodiesterase followed by the subsequent generation of diacylglycerol and a soluble phosphoinositol-oligosaccharide (inositol-phosphoglycan; (Ose)(n)InsP) moiety could be part of the signal-transduction mechanism linking PRL receptors to their biological effects in granulosa cells. To test this hypothesis, we examined the effect of PRL and purified (Ose)(n)InsP moiety (from rat liver membranes) on granulosa cell 3beta-hydroxysteroid dehydrogenase/DELTA5-4 isomerase (3beta-HSD) enzyme activity. Results presented show that, in FSH-primed granulosa cells, PRL (40 nM) and (Ose)(n)InsP (5 muM) prevented gonadotropin-stimulated 3beta-HSD activity. Furthermore, in undifferentiated granulosa cells where PRL receptors are absent, no effect of the hormone on 3beta-HSD activity could be observed, whereas (Ose)(n)InsP (1-10 muM) inhibited enzyme activity in a dose-dependent manner. | en_US |
dc.language | eng | en_US |
dc.relation.ispartof | European journal of biochemistry (Print) | en_US |
dc.source | European journal of biochemistry [ISSN 0014-2956], v. 216 (3), p. 747-755, (Septiembre 1993) | en_US |
dc.subject | 32 Ciencias médicas | en_US |
dc.subject | 2403 Bioquímica | en_US |
dc.subject.other | Protein-Kinase-C | en_US |
dc.subject.other | Gonadotropin-Releasing Hormone | en_US |
dc.subject.other | Follicle-Stimulating-Hormone | en_US |
dc.subject.other | Human Chorionic-Gonadotropin | en_US |
dc.subject.other | Hepatic Plasma-Membranes | en_US |
dc.subject.other | Isolated Rat Hepatocytes | en_US |
dc.subject.other | Mimics Insulin Action | en_US |
dc.subject.other | Nerve Growth-Factor | en_US |
dc.subject.other | Polar Head Group | en_US |
dc.subject.other | Ovarian Granulosa | en_US |
dc.title | Does oligosaccharide‐phosphatidylinositol (glycosyl‐phosphatidylinositol) hydrolysis mediate prolactin signal transduction in granulosa cells? | en_US |
dc.type | info:eu-repo/semantics/Article | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.1111/j.1432-1033.1993.tb18194.x | en_US |
dc.identifier.pmid | 8404893 | - |
dc.identifier.scopus | 0027430799 | - |
dc.identifier.isi | A1993LX85200006 | - |
dc.contributor.orcid | #NODATA# | - |
dc.contributor.orcid | #NODATA# | - |
dc.contributor.orcid | #NODATA# | - |
dc.contributor.orcid | #NODATA# | - |
dc.contributor.orcid | #NODATA# | - |
dc.contributor.orcid | #NODATA# | - |
dc.contributor.orcid | #NODATA# | - |
dc.contributor.orcid | #NODATA# | - |
dcterms.isPartOf | European Journal Of Biochemistry | - |
dcterms.source | European Journal Of Biochemistry[ISSN 0014-2956],v. 216 (3), p. 747-755 | - |
dc.contributor.authorscopusid | 7004158812 | - |
dc.contributor.authorscopusid | 35936487800 | - |
dc.contributor.authorscopusid | 57198495832 | - |
dc.contributor.authorscopusid | 8681043500 | - |
dc.contributor.authorscopusid | 7003526778 | - |
dc.contributor.authorscopusid | 7003810011 | - |
dc.contributor.authorscopusid | 55399368600 | - |
dc.contributor.authorscopusid | 7003806034 | - |
dc.description.lastpage | 755 | en_US |
dc.identifier.issue | 3 | - |
dc.description.firstpage | 747 | en_US |
dc.relation.volume | 216 | en_US |
dc.investigacion | Ciencias de la Salud | en_US |
dc.type2 | Artículo | en_US |
dc.identifier.wos | WOS:A1993LX85200006 | - |
dc.contributor.daisngid | 1127140 | - |
dc.contributor.daisngid | 1533885 | - |
dc.contributor.daisngid | 3844163 | - |
dc.contributor.daisngid | 7986161 | - |
dc.contributor.daisngid | 136721 | - |
dc.contributor.daisngid | 128315 | - |
dc.contributor.daisngid | 31449715 | - |
dc.contributor.daisngid | 329218 | - |
dc.contributor.daisngid | 384944 | - |
dc.contributor.daisngid | 26350 | - |
dc.contributor.daisngid | 1664323 | - |
dc.identifier.investigatorRID | Q-9437-2016 | - |
dc.identifier.investigatorRID | A-5187-2011 | - |
dc.identifier.investigatorRID | K-5125-2014 | - |
dc.identifier.investigatorRID | K-5709-2014 | - |
dc.identifier.external | 22660304 | - |
dc.description.numberofpages | 9 | en_US |
dc.utils.revision | Sí | en_US |
dc.contributor.wosstandard | WOS:FANJUL, LF | - |
dc.contributor.wosstandard | WOS:MARRERO, I | - |
dc.contributor.wosstandard | WOS:GONALEZ, J | - |
dc.contributor.wosstandard | WOS:QUINTANA, J | - |
dc.contributor.wosstandard | WOS:SANTANA, P | - |
dc.contributor.wosstandard | WOS:ESTEVEZ, F | - |
dc.contributor.wosstandard | WOS:MATO, JM | - |
dc.contributor.wosstandard | WOS:DEGALARRETA, CMR | - |
dc.date.coverdate | Septiembre 1993 | en_US |
dc.identifier.ulpgc | Sí | en_US |
dc.contributor.buulpgc | BU-MED | en_US |
item.grantfulltext | open | - |
item.fulltext | Con texto completo | - |
crisitem.author.dept | Departamento de Bioquímica y Biología Molecular, Fisiología, Genética e Inmunología | - |
crisitem.author.dept | GIR IUIBS: Bioquímica | - |
crisitem.author.dept | IU de Investigaciones Biomédicas y Sanitarias | - |
crisitem.author.dept | Departamento de Bioquímica y Biología Molecular, Fisiología, Genética e Inmunología | - |
crisitem.author.dept | GIR IUIBS: Bioquímica | - |
crisitem.author.dept | IU de Investigaciones Biomédicas y Sanitarias | - |
crisitem.author.dept | Departamento de Bioquímica y Biología Molecular, Fisiología, Genética e Inmunología | - |
crisitem.author.dept | GIR IUIBS: Bioquímica | - |
crisitem.author.dept | IU de Investigaciones Biomédicas y Sanitarias | - |
crisitem.author.dept | Departamento de Bioquímica y Biología Molecular, Fisiología, Genética e Inmunología | - |
crisitem.author.orcid | 0009-0000-1982-055X | - |
crisitem.author.orcid | 0000-0003-3732-9929 | - |
crisitem.author.orcid | 0000-0001-8225-4538 | - |
crisitem.author.orcid | 0000-0002-9728-2774 | - |
crisitem.author.parentorg | IU de Investigaciones Biomédicas y Sanitarias | - |
crisitem.author.parentorg | IU de Investigaciones Biomédicas y Sanitarias | - |
crisitem.author.parentorg | IU de Investigaciones Biomédicas y Sanitarias | - |
crisitem.author.fullName | Fanjul Rodríguez, Luisa Fernanda | - |
crisitem.author.fullName | Marrero Arencibia, María Isabel | - |
crisitem.author.fullName | Quintana Aguiar, José Martín | - |
crisitem.author.fullName | Estévez Rosas, Francisco Jesús | - |
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