Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/53522
DC FieldValueLanguage
dc.contributor.authorQuintana, Jen_US
dc.contributor.authorFanjul, Luisa F.en_US
dc.contributor.authorMarrero, Isabelen_US
dc.contributor.authorEstevez, F.en_US
dc.contributor.authorGonzalez, J.en_US
dc.contributor.authorSantana, Pinoen_US
dc.contributor.authorDe Galarreta, C. M.Ruizen_US
dc.contributor.otherQuintana, Jose-
dc.contributor.otherMarrero-Arencibia, Isabel-
dc.contributor.otherEstevez, Francisco-
dc.date.accessioned2019-02-04T17:02:14Z-
dc.date.available2019-02-04T17:02:14Z-
dc.date.issued1993en_US
dc.identifier.issn0021-9541en_US
dc.identifier.urihttp://hdl.handle.net/10553/45311-
dc.description.abstractIn the present investigation, a hCG sensitive glycosyl-phosphatidylinositol (GPI) was isolated from cultured rat granulosa cells obtained from the ovaries of diethylstilbestrol (DES) implanted immature rats. The inositol-phosphoglycan (IPG) moiety of the GPI-lipid contains galactose, glucosamine, and myoinositol as demonstrated by metabolic labelling of granulosa cells for different time periods (5-96 h) with [H-3]galactose, [H-3]glucosamine, or [H-3]myoinositol and treatment of the purified [H-3]GPI with phosphatidylinositol-specific phospholipase C. Labelling equilibrium of the GPI-lipid was achieved after 24 h ([H-3]galactose and [H-3]myoinositol) or 72 h ([H-3]glucosamine) incubation, whereas incorporation of other labelled carbohydrates tested ([H-3]galactosamine, [H-3]mannose, and [H-3]sorbitol) was negligible throughout the time period studied. The glucosamine C-1 appears to be linked through a glycosidic bond to the myoinositol molecule of the IPG moiety as revealed by the generation of phosphatidylinositol (PtdIns) after nitrous acid deamination of dual labelled ([H-3]glucosamine/[C-14]palmitate or [H-3]glucosamine/[C-14]myristate) glycosyl-phosphatidylinositol. To investigate the fatty acid composition of the diacylglycerol (DAG) backbone of the GPI, granulosa cells were also labelled (5-72 hr) with [C-14]linoleate, [H-3]myristate, [H-3}-oleate, [H-3]palmitate, or [H-3]stearate and the radioactivity associated with the purified glycosyl-phosphatidylinositol determined. Incorporation of [H-3]palmitate and [H-3]myristate into the GPI-lipid peaked after 8 h and 24 h of labelling, respectively, and both fatty acids were partially released after PLA, treatment of the dual labelled ([H-3]glucosamine/[C-14]palmitate or [H-3]glucosamine/[C-14]myristate) GPI. In parallel experiments no significant incorporation of labelled stearate, oleate, or linoleic acid into the DAG backbone of the glycosyl-phosphatidylinositol could be detected. Granulosa cells were also labelled with [H-3]glucosamine in the presence of FSH (30 ng/ml), cholera toxin (1 mug/ml), or the membrane permeable cAMP analog (bUt)2cAMP (1 mM). Time related increases in GPI-labelling were apparent after 48 h and reached a maximum level (3-, 5-, and 7-fold for FSH, CT, and (bUt)2cAMP, respectively) after 72 h in culture. In another set of experiments, granulosa cells were labelled for 72 h with [H-3]glucosamine in the presence of (but)2cAMP (1 mM), TPA (10(-7) M), or combination thereof. The effect of treatment with the membrane permeable cAMP analog on GPI labelling was prevented in the presence of TPA, whereas no differences in [H-3]GPI content could be observed in untreated granulosa cells or cells cultured in the presence of the protein kinase C-activating phorbol ester alone. In cells differentiated with FSH (30 ng/ml for 3 days) to induce LH receptors, treatment with hCG (100 ng/ml) induced a rapid (60 sec) and transient (5 min) decrease in the GPI content, whereas no effect of the hormone on undifferentiated granulosa cells could be observed. The rapid effect elicited by hCG on GPI content and turnover may be an early transduction mechanism involved in the biological effects of LH/hCG in differentiated granulosa cells.en_US
dc.languageengen_US
dc.relation.ispartofJournal of Cellular Physiologyen_US
dc.sourceJournal Of Cellular Physiology [ISSN 0021-9541], v. 155 (2), p. 273-281en_US
dc.subject32 Ciencias médicasen_US
dc.subject2411 Fisiología humanaen_US
dc.subject2407 Biología celularen_US
dc.subject.otherProtein-Kinase-Cen_US
dc.subject.otherPolar Head Groupen_US
dc.subject.otherLuteinizing-Hormoneen_US
dc.subject.otherPyruvate-Dehydrogenaseen_US
dc.subject.otherMembrane Anchoren_US
dc.subject.otherPhosphoinositide Metabolismen_US
dc.subject.otherPhospho-Oligosaccharideen_US
dc.subject.otherCoordinated Regulationen_US
dc.subject.otherThy-1 Glycoproteinen_US
dc.subject.otherOvarian-Cellsen_US
dc.titleFollicle‐stimulating hormone and human chorionic gonadotropin induced changes in granulosa cell glycosyl‐phosphatidylinositol concentrationen_US
dc.typeinfo:eu-repo/semantics/Articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1002/jcp.1041550208en_US
dc.identifier.scopus0027251642-
dc.identifier.isiA1993KZ50100007-
dcterms.isPartOfJournal Of Cellular Physiology-
dcterms.sourceJournal Of Cellular Physiology[ISSN 0021-9541],v. 155 (2), p. 273-281-
dc.contributor.authorscopusid7004158812-
dc.contributor.authorscopusid35936487800-
dc.contributor.authorscopusid7003810011-
dc.contributor.authorscopusid57198495832-
dc.contributor.authorscopusid8681043500-
dc.contributor.authorscopusid7003526778-
dc.contributor.authorscopusid6506605794-
dc.description.lastpage281en_US
dc.identifier.issue2-
dc.description.firstpage273en_US
dc.relation.volume155en_US
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.identifier.wosWOS:A1993KZ50100007-
dc.contributor.daisngid1127140-
dc.contributor.daisngid3844163-
dc.contributor.daisngid1533885-
dc.contributor.daisngid384944-
dc.contributor.daisngid14398846-
dc.contributor.daisngid128315-
dc.contributor.daisngid31449715-
dc.contributor.daisngid329218-
dc.contributor.daisngid1664323-
dc.identifier.investigatorRIDK-5709-2014-
dc.identifier.investigatorRIDQ-9437-2016-
dc.identifier.investigatorRIDK-5125-2014-
dc.description.numberofpages9en_US
dc.utils.revisionen_US
dc.contributor.wosstandardWOS:FANJUL, LF-
dc.contributor.wosstandardWOS:MARRERO, I-
dc.contributor.wosstandardWOS:ESTEVEZ, F-
dc.contributor.wosstandardWOS:GONZALEZ, J-
dc.contributor.wosstandardWOS:QUINTANA, J-
dc.contributor.wosstandardWOS:SANTANA, P-
dc.contributor.wosstandardWOS:DEGALARRETA, CMR-
dc.date.coverdateMayo 1993en_US
dc.identifier.ulpgcen_US
dc.contributor.buulpgcBU-MEDen_US
item.fulltextSin texto completo-
item.grantfulltextnone-
crisitem.author.deptSeñalización Intracelular y Expresión Génica-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Bioquímica y Biología Molecular, Fisiología, Genética e Inmunología.-
crisitem.author.deptDepartamento de Bioquímica y Biología Molecular, Fisiología, Genética e Inmunología.-
crisitem.author.deptBioquímica Farmacológica-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Bioquímica y Biología Molecular, Fisiología, Genética e Inmunología.-
crisitem.author.deptBioquímica Farmacológica-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Bioquímica y Biología Molecular, Fisiología, Genética e Inmunología.-
crisitem.author.deptBioquímica Farmacológica-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Bioquímica y Biología Molecular, Fisiología, Genética e Inmunología.-
crisitem.author.orcid0000-0003-3732-9929-
crisitem.author.orcid0000-0002-9728-2774-
crisitem.author.orcid0000-0002-9728-2774-
crisitem.author.orcid0000-0001-8225-4538-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.fullNameFanjul Rodríguez, Luisa Fernanda-
crisitem.author.fullNameMarrero Arencibia, María Isabel-
crisitem.author.fullNameEstévez Rosas, Francisco Jesús-
crisitem.author.fullNameEstévez Rosas, Francisco Jesús-
crisitem.author.fullNameQuintana Aguiar, José Martín-
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