Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/52898
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dc.contributor.authorMorales-Alamo, Daviden_US
dc.contributor.authorGustavo Ponce-Gonzalez, Jesusen_US
dc.contributor.authorGuadalupe-Grau, Ameliaen_US
dc.contributor.authorRodriguez-Garcia, Lorenaen_US
dc.contributor.authorSantana, Alfredoen_US
dc.contributor.authorCusso, Roseren_US
dc.contributor.authorGuerrero, Marioen_US
dc.contributor.authorDorado, Ceciliaen_US
dc.contributor.authorGuerra, Borjaen_US
dc.contributor.authorCalbet, Jose A. L.en_US
dc.contributor.otherCecilia, Dorado-Garcia-
dc.contributor.otherPonce Gonzalez, Jesus Gustavo-
dc.contributor.otherMorales-Alamo, David-
dc.contributor.otherGuadalupe-Grau, Amelia-
dc.contributor.otherGuerra, Borja-
dc.contributor.otherCalbet, Jose A-
dc.date.accessioned2019-02-04T14:14:57Z-
dc.date.available2019-02-04T14:14:57Z-
dc.date.issued2013en_US
dc.identifier.issn8750-7587en_US
dc.identifier.urihttp://hdl.handle.net/10553/52898-
dc.description.abstractMorales-Alamo D, Ponce-Gonzalez JG, Guadalupe-Grau A, Rodriguez-Garcia L, Santana A, Cusso R, Guerrero M, Dorado C, Guerra B, Calbet JA. Critical role for free radicals on sprint exercise-induced CaMKII and AMPK alpha phosphorylation in human skeletal muscle. J Appl Physiol 114: 566-577, 2013. First published January 3, 2013; doi:10.1152/japplphysiol.01246.2012.-The extremely high energy demand elicited by sprint exercise is satisfied by an increase in O-2 consumption combined with a high glycolytic rate, leading to a marked lactate accumulation, increased AMP-to-ATP ratio, and reduced NAD(+)/NADH.H+ and muscle pH, which are accompanied by marked Thr(172) AMP-activated protein kinase (AMPK)-alpha phosphorylation during the recovery period by a mechanism not fully understood. To determine the role played by reactive nitrogen and oxygen species (RNOS) on Thr(172)-AMPK alpha phosphorylation in response to cycling sprint exercise, nine voluntary participants performed a single 30-s sprint (Wingate test) on two occasions: one 2 h after the ingestion of placebo and another after the intake of antioxidants (alpha-lipoic acid, vitamin C, and vitamin E) in a double-blind design. Vastus lateralis muscle biopsies were obtained before, immediately postsprint, and 30 and 120 min postsprint. Performance and muscle metabolism were similar during both sprints. The NAD(+)-to-NADH.H+ ratio was similarly reduced (84%) and the AMP-to-ATP ratio was similarly increased (x21-fold) immediately after the sprints. Thr(286) Ca2+/cal-modulin-dependent protein kinase II (CaMKII) and Thr(172)-AMPK alpha phosphorylations were increased after the control sprint (with placebo) but not when the sprints were preceded by the ingestion of antioxidants. Ser(485)-AMPK alpha(1)/Ser(491)-AMPK alpha(2) phosphorylation, a known inhibitory mechanism of Thr(172)-AMPK alpha phosphorylation, was increased only with antioxidant ingestion. In conclusion, RNOS play a crucial role in AMPK-mediated signaling after sprint exercise in human skeletal muscle. Antioxidant ingestion 2 h before sprint exercise abrogates the Thr(172)-AMPK alpha phosphorylation response observed after the ingestion of placebo by reducing CaMKII and increasing Ser(485)-AMPK alpha(1)/Ser(491)-AMPK alpha(2) phosphorylation. Sprint performance, muscle metabolism, and AMP-to-ATP and NAD(+)-to-NADH.H+ ratios are not affected by the acute ingestion of antioxidants.en_US
dc.languagespaen_US
dc.publisher8750-7587
dc.relationIntegracion de Los Grupos de la Obesidad y El Síndrome Metabólico ....en_US
dc.relation.ispartofJournal of Applied Physiologyen_US
dc.sourceJournal Of Applied Physiology[ISSN 8750-7587],v. 114 (5), p. 566-577en_US
dc.subject.otherActivated Protein-Kinase
dc.subject.otherNf-Kappa-B
dc.subject.otherLipoic Acid
dc.subject.otherOxidative Stress
dc.subject.otherMitochondrial Biogenesis
dc.subject.otherFatty-Acid
dc.subject.otherGlucose-Uptake
dc.subject.otherMessenger-Rna
dc.subject.otherFa Uptake
dc.subject.otherVitamin-E
dc.titleCritical role for free radicals on sprint exercise-induced CaMKII and AMPK alpha phosphorylation in human skeletal muscleen_US
dc.typeinfo:eu-repo/semantics/Articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1152/japplphysiol.01246.2012en_US
dc.identifier.isi000315680700005-
dcterms.isPartOfJournal Of Applied Physiology
dcterms.sourceJournal Of Applied Physiology[ISSN 8750-7587],v. 114 (5), p. 566-577
dc.description.lastpage577en_US
dc.identifier.issue5-
dc.description.firstpage566en_US
dc.relation.volume114en_US
dc.type2Artículoen_US
dc.identifier.wosWOS:000315680700005-
dc.contributor.daisngid1232764-
dc.contributor.daisngid1264657-
dc.contributor.daisngid7526316-
dc.contributor.daisngid861361-
dc.contributor.daisngid2486534-
dc.contributor.daisngid31470516-
dc.contributor.daisngid1230811-
dc.contributor.daisngid496197-
dc.contributor.daisngid530587-
dc.contributor.daisngid629383-
dc.contributor.daisngid909211-
dc.contributor.daisngid90295-
dc.identifier.investigatorRIDB-4586-2010-
dc.identifier.investigatorRIDH-9500-2015-
dc.identifier.investigatorRIDD-2683-2009-
dc.identifier.investigatorRIDH-4961-2016-
dc.identifier.investigatorRIDG-9739-2015-
dc.identifier.investigatorRIDH-6693-2015-
dc.contributor.wosstandardWOS:Morales-Alamo, D-
dc.contributor.wosstandardWOS:Ponce-Gonzalez, JG-
dc.contributor.wosstandardWOS:Guadalupe-Grau, A-
dc.contributor.wosstandardWOS:Rodriguez-Garcia, L-
dc.contributor.wosstandardWOS:Santana, A-
dc.contributor.wosstandardWOS:Cusso, R-
dc.contributor.wosstandardWOS:Guerrero, M-
dc.contributor.wosstandardWOS:Dorado, C-
dc.contributor.wosstandardWOS:Guerra, B-
dc.contributor.wosstandardWOS:Calbet, JAL-
dc.date.coverdateMarzo 2013en_US
dc.identifier.ulpgces
item.fulltextSin texto completo-
item.grantfulltextnone-
crisitem.author.deptDepartamento de Ciencias Clínicas-
crisitem.author.deptRendimiento humano, ejercicio físico y salud-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Educación Física-
crisitem.author.deptFarmacología Molecular y Traslacional-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Ciencias Clínicas-
crisitem.author.deptRendimiento humano, ejercicio físico y salud-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Educación Física-
crisitem.author.orcid0000-0001-6616-6810-
crisitem.author.orcid0000-0003-4355-5682-
crisitem.author.orcid0000-0002-9215-6234-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.fullNameSantana Rodríguez, Alfredo-
crisitem.author.fullNameDorado García, Cecilia-
crisitem.author.fullNameGuerra Hernández, Carlos Borja-
crisitem.author.fullNameLópez Calbet, José Antonio-
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