Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/113219
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dc.contributor.authorCarvalho, Isabelen_US
dc.contributor.authorChenouf, Nadia Sofíaen_US
dc.contributor.authorGonzález Martín, Margarita Rosaen_US
dc.contributor.authorCorbera Sánchez, Juan Albertoen_US
dc.contributor.authorTejedor-Junco, María Teresaen_US
dc.contributor.authorIgrejas, Gilbertoen_US
dc.contributor.authorTorres, Carmenen_US
dc.contributor.authorPoeta, Patriciaen_US
dc.date.accessioned2022-01-12T12:47:18Z-
dc.date.available2022-01-12T12:47:18Z-
dc.date.issued2020en_US
dc.identifier.urihttp://hdl.handle.net/10553/113219-
dc.description.abstractAntimicrobial resistance is a serious public health problem worldwide. Data on the occurrence of multidrug resistant Escherichia coli in camels are lacking. The aim of this work was to determine the carriage rate of ESBL-producing E. coli as well as their genetic lineages in camels from Fuerteventura and Gran Canaria (Canary Islands), in Spain. Faecal samples were recovered from 58 apparently healthy camels from Gran Canaria (n=32) and Fuerteventura (n=26) during July 2019. They were seeded on MacConkey agar no supplemented (MC) and supplemented (MC+CTX) with cefotaxime (2μg/ml) for E. coli recovery. Antimicrobial susceptibility was performed by disk-diffusion test (CLSI, 2018). The presence of blaCTX-M (different groups), blaSHV, blaTEM, blaKPC, blaOXA-1 and blaOXA-48 genes, as well as mcr-1 (colistin resistance), tetA/tetB (tetracycline resistance) and int1 gene (integrase of class 1 integrons), was tested by PCR/sequencing. Furthermore, phylogenetic groups and MLST were determined by specific-PCR and sequencing. E. coli strains were isolated from all the 58 camels (100%): 8 recovered from MC+CTX and 58 from MC. Most of them were resistant to one or two antibiotics, so we had focused our research in the 5 multidrug resistant E. coli isolates. ESBL producing-E. coli were detected in 2 camels samples (4% of the total animals tested) with the same resistant genotype: blaCTX-M-15, blaTEM-1, tetA, tetB, int1. ESBL-positive isolates were both ascribed to phylogenetic group A and to the sequence type ST3018, while the ESBL negative isolates were phylogroup B1 (3 isolates). To our knowledge, this is the first report related to E. coli in camels from Canary Islands. Camels can be a source of ESBL genes, containing the widespread blaCTX-M-15 gene in isolates of lineage ST3018-A. Due to the participation of camels in touristic activities in the region, the potential transference of ESBL-positive bacteria between humans and animals could easily happened and should be further monitoreden_US
dc.languageengen_US
dc.subject310905 Microbiologíaen_US
dc.subject.otherAntimicrobial resistanceen_US
dc.subject.otherExtended-spectrum β-lactamasesen_US
dc.subject.otherEscherichia colien_US
dc.titleFirst report of Escherichia coli producing extended-spectrum Beta-lactamases (ESBL) from camels in Canary Islands.en_US
dc.typeinfo:eu-repo/semantics/lectureen_US
dc.typeLectureen_US
dc.relation.conferenceXVI Congreso Internacional Veterinario Montenegro (2020)en_US
dc.investigacionCiencias de la Saluden_US
dc.type2Ponenciaen_US
dc.utils.revisionen_US
dc.identifier.ulpgcen_US
dc.contributor.buulpgcBU-VETen_US
dc.contributor.buulpgcBU-VETen_US
dc.contributor.buulpgcBU-VETen_US
dc.contributor.buulpgcBU-VETen_US
item.fulltextSin texto completo-
item.grantfulltextnone-
crisitem.author.deptGIR IUIBS: Trypanosomosis, Resistencia a Antibióticos y Medicina Animal-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Ciencias Clínicas-
crisitem.author.deptGIR IUIBS: Trypanosomosis, Resistencia a Antibióticos y Medicina Animal-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.deptGIR IUIBS: Trypanosomosis, Resistencia a Antibióticos y Medicina Animal-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Ciencias Clínicas-
crisitem.author.orcid0000-0002-4457-2321-
crisitem.author.orcid0000-0001-7812-2065-
crisitem.author.orcid0000-0003-2387-1426-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.fullNameGonzález Martín, Margarita Rosa-
crisitem.author.fullNameCorbera Sánchez, Juan Alberto-
crisitem.author.fullNameTejedor Junco, María Teresa-
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