Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/7889
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dc.contributor.authorMoreno-Indias, Isabelen_US
dc.contributor.authorDodds, Alister W.en_US
dc.contributor.authorArgueello, Anastasioen_US
dc.contributor.authorCastro, Noemien_US
dc.contributor.authorSim, Robert B.en_US
dc.contributor.otherMoreno-Indias, Isabel-
dc.contributor.otherCastro, Noemi-
dc.contributor.otherMoreno-Indias, Isabel-
dc.contributor.otherSim, Robert-
dc.contributor.otherArguello, Anastasio-
dc.contributor.otherDodds, Alister William-
dc.date.accessioned2012-08-17T09:13:55Z-
dc.date.accessioned2018-03-08T13:18:34Z-
dc.date.available2012-08-17T09:13:55Z-
dc.date.available2018-03-08T13:18:34Z-
dc.date.issued2012en_US
dc.identifierhttp://dx.doi.org/10.1186/1746-6148-8-91-
dc.identifier.issn1746-6148en_US
dc.identifier.otherScopus-
dc.identifier.urihttp://hdl.handle.net/10553/7889-
dc.description.abstractBackground: The aim of the present study was to develop a haemolytic assay for the study of the complement system in dairy goats (Capra aegagrus hircus) and to characterize the major goat complement system proteins. Results: The commonly used sheep erythrocyte sensitized with rabbit antibodies were not sensitive to lysis by goat serum, but the combination of human red blood cells (RBC) plus rabbit antibodies was the best option found for goat complement assay. A buffer based on HEPES instead of the classical veronal (barbitone) was developed. Three proteins were isolated: factor H, C1q and C3 and these were compared with the corresponding human proteins. A novel affinity chromatography technique was developed for isolation of factor H. Conclusions: Human RBC plus rabbit antibodies were a suitable option for haemolytic assays. The isolated proteins are similar to the human counterparts.en_US
dc.languagespaen_US
dc.relation.ispartofBMC Veterinary Researchen_US
dc.sourceBmc Veterinary Research[ISSN 1746-6148],v. 8en_US
dc.subject3109 Ciencias veterinariasen_US
dc.subject3104 Producción Animalen_US
dc.subject.otherGoaten_US
dc.subject.otherComplement systemen_US
dc.subject.otherC3en_US
dc.subject.otherC1qen_US
dc.subject.otherFactor Hen_US
dc.titleThe complement system of the goat: haemolytic assays and isolation of major proteinsen_US
dc.typeinfo:eu-repo/semantics/Articleen_US
dc.typeinfo:eu-repo/semantics/Articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1186/1746-6148-8-91en_US
dc.identifier.scopus84862661585-
dc.identifier.isi000307249500001-
dcterms.isPartOfBmc Veterinary Research-
dcterms.sourceBmc Veterinary Research[ISSN 1746-6148],v. 8-
dc.date.updated2012-08-10T05:21:59Z-
dc.description.versionPeer Reviewed-
dc.language.rfc3066en-
dc.rights.holderIsabel Moreno-Indias et al.; licensee BioMed Central Ltd.-
dc.compliance.driver1-
dc.contributor.authorscopusid24332457100-
dc.contributor.authorscopusid7005260746-
dc.contributor.authorscopusid6701710018-
dc.contributor.authorscopusid57200208399-
dc.contributor.authorscopusid7101832009-
dc.identifier.crisid-;-;1736;6869;--
dc.identifier.eissn1746-6148-
dc.relation.volume8en_US
dc.investigacionCiencias de la Saluden_US
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess-
dc.type2Artículoen_US
dc.identifier.wosWOS:000307249500001-
dc.contributor.daisngid1065675-
dc.contributor.daisngid545538-
dc.contributor.daisngid298051-
dc.contributor.daisngid330531-
dc.contributor.daisngid18883-
dc.identifier.investigatorRIDE-8907-2011-
dc.identifier.investigatorRIDF-9621-2016-
dc.identifier.investigatorRIDB-6595-2017-
dc.identifier.investigatorRIDA-1354-2008-
dc.identifier.investigatorRIDB-4493-2010-
dc.identifier.investigatorRIDNo ID-
dc.utils.revisionen_US
dc.contributor.wosstandardWOS:Moreno-Indias, I-
dc.contributor.wosstandardWOS:Dodds, AW-
dc.contributor.wosstandardWOS:Arguello, A-
dc.contributor.wosstandardWOS:Castro, N-
dc.contributor.wosstandardWOS:Sim, RB-
dc.date.coverdateJunio 2012en_US
dc.identifier.supplementhttp://dx.doi.org/10.1186/1746-6148-8-91-
dc.identifier.supplement-;-;1736;6869;--
dc.identifier.ulpgces
dc.description.sjr0,743
dc.description.jcr1,861
dc.description.sjrqQ1
dc.description.jcrqQ1
dc.description.scieSCIE
item.fulltextCon texto completo-
item.grantfulltextopen-
crisitem.author.deptGIR IUSA-ONEHEALTH 4. Producción y Biotecnología Animal-
crisitem.author.deptIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.deptDepartamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.deptGIR IUSA-ONEHEALTH 4. Producción y Biotecnología Animal-
crisitem.author.deptIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.deptDepartamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.orcid0000-0002-4426-0678-
crisitem.author.orcid0000-0002-3026-2031-
crisitem.author.parentorgIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.parentorgIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.fullNameArgüello Henríquez, Anastasio-
crisitem.author.fullNameCastro Navarro, Noemí-
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