Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/77936
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dc.contributor.authorWall, Samantha K.en_US
dc.contributor.authorHernández Castellano, Lorenzo Enriqueen_US
dc.contributor.authorAhmadpour, Amiren_US
dc.contributor.authorBruckmaier, Rupert M.en_US
dc.contributor.authorWellnitz, Olgaen_US
dc.date.accessioned2021-03-04T13:49:49Z-
dc.date.available2021-03-04T13:49:49Z-
dc.date.issued2016en_US
dc.identifier.issn0022-0302en_US
dc.identifier.urihttp://hdl.handle.net/10553/77936-
dc.description.abstractBacteria invading the mammary gland can cause pathogen-dependent differences in the permeability of the blood-milk barrier leading to the differential paracellular transfer of blood and milk components. Glucocorticoids such as prednisolone (PRED) are known to increase the integrity of the blood-milk barrier and quickly restore the decreased milk quality associated with mastitis. The objective of this study was to examine the effect of intramammary PRED on the differential permeability of the blood-milk barrier during mastitis induced by lipopolysaccharide (LPS) from Escherichia coli or lipoteichoic acid (LTA) from Staphylococcus aureus. Thirty-one dairy cows, divided into 6 groups, were injected via a teat canal with LPS, LTA, LPS and PRED, LTA and PRED, saline (control), or PRED. Milk and blood samples were collected 0 to 8 h after challenge and analyzed for somatic cell count, IgG, serum albumin, and lactate dehydrogenase in milk, or α-lactalbumin in plasma. Somatic cell count was similarly elevated in LPS- and LTA-challenged quarters and was reduced to control quarter levels only in LTA-challenged quarters with PRED administration. Lactate dehydrogenase activity was highly elevated in LPS quarters and only slightly elevated in LTA quarters, but decreased to control quarter levels with PRED administration. For serum albumin and IgG, only LPS quarters showed an elevation in concentration and PRED treatment reduced the concentration to control quarter level. We found no differences in α-lactalbumin concentrations in plasma in PRED-treated cows compared with cows that only received LPS or LTA. In conclusion, the pathogen-specific appearance of blood constituents in milk during mastitis demonstrates a differential activation of the blood-milk barrier that, in turn, can be manipulated by intramammary glucocorticoids. The results show that the administration of PRED during mastitis increases the blood-milk barrier integrity but has implications in reducing the transfer of IgG that specifically occurs during E. coli mastitis. In addition, it can also reduce the number of migrating immune cells dependent on the mastitis-inducing pathogen. Potential effects of PRED on the cure of naturally occurring mastitis have to be taken into consideration.en_US
dc.languageengen_US
dc.relation.ispartofJournal of Dairy Scienceen_US
dc.sourceJournal of Dairy Science [ISSN 0022-0302], v. 99(9), p. 7544-7553en_US
dc.subject3104 Producción Animalen_US
dc.subject.otherMastitisblood-milk barrieren_US
dc.subject.otherGlucocorticoiden_US
dc.subject.otherEndotoxinen_US
dc.titleDifferential glucocorticoid-induced closure of the blood-milk barrier during lipopolysaccharide- and lipoteichoic acid-induced mastitis in dairy cowsen_US
dc.typeinfo:eu-repo/semantics/Articleen_US
dc.typearticleen_US
dc.identifier.doi10.3168/jds.2016-11093en_US
dc.identifier.issue9-
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.utils.revisionen_US
dc.identifier.ulpgcNoen_US
dc.contributor.buulpgcBU-VETen_US
dc.description.sjr1,304
dc.description.jcr2,474
dc.description.sjrqQ1
dc.description.jcrqQ1
dc.description.scieSCIE
item.grantfulltextopen-
item.fulltextCon texto completo-
crisitem.author.deptGIR Producción y Biotecnología Animal-
crisitem.author.deptIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.orcid0000-0003-2729-0434-
crisitem.author.parentorgIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.fullNameHernández Castellano, Lorenzo Enrique-
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