Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/74271
Título: Rapid differential diagnosis of Mycoplasma agalactiae and Mycoplasma bovis based on a multiplex-PCR and a PCR-RFLP
Autores/as: Foddai, A.
Idini, G.
Fusco, M.
Rosa, N.
de la Fe, C.
Zinellu, S.
Corona, L.
Tola, S.
Clasificación UNESCO: 3109 Ciencias veterinarias
Palabras clave: Mycoplasma Agalactiae
Mycoplasma Bovis
Multiplex-PCR
PCR-RFLP
Ma(Mb)-Mp81 Gene
Fecha de publicación: 2005
Publicación seriada: Molecular and Cellular Probes 
Resumen: The membrane-protein 81 gene (mb-mp81) of Mycoplasma bovis was cloned, sequenced and compared to membrane-protein 81 gene (ma-mp81) of Mycoplasma agalactine. After alignment of both sequences, specific primers pairs were designed from variable or unchanging nucleotide segments. In this study, we describe the development and optimization of a multiplex-PCR (MPCR) for the rapid detection of M. agalactiae and M. bovis strains. In addition, a simple and rapid PCR-restriction fragment length polymorphism (RFLP) assay, using the restriction enzymes AluI. DraI, RsaI and XbaI, is described to distinguish between both species. The results suggest that MPCR and PCR-RFLP assays could be used as an alternative method in routine diagnosis for rapid and specific simultaneous detection of M. agalactiae and M. bovis.
URI: http://hdl.handle.net/10553/74271
ISSN: 0890-8508
DOI: 10.1016/j.mcp.2004.11.007
Fuente: Molecular and Cellular Probes [ISSN 0890-8508], v. 19 (3), p. 207-212, (Junio 2005)
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