Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/73103
Título: Lymphatic endothelial cell immunohistochemical markers for evaluation of the intestinal lymphatic vasculature in dogs with chronic inflammatory enteropathy
Autores/as: Wennogle, Sara A.
Priestnall, Simon L.
Suárez Bonnet, Alejandro 
Soontararak, Sirikul
Webb, Craig B.
Clasificación UNESCO: 310907 Patología
240118 Mamíferos
2401 Biología animal (zoología)
Palabras clave: Canine
Enteropathy
Immunolabeling
Lacteals.
Fecha de publicación: 2019
Publicación seriada: Journal of Veterinary Internal Medicine 
Resumen: Background: Lymphatic endothelial cell (LEC) immunohistochemical markers have identified intestinal lymphatic vasculature abnormalities in humans with inflammatory bowel disease, but have not been used to evaluate intestinal lymphatic vasculature in a group of dogs with chronic inflammatory enteropathy (CIE). Objectives: To utilize LEC markers to identify and measure intestinal lymphatic vasculature in endoscopic biopsy samples of CIE dogs. To evaluate whether measured lymphatic vasculature variables correlate with serum albumin concentrations. Animals: Twenty-four dogs with CIE; n = 13, serum albumin concentration <2.5 g/dL (CIE-protein-losing enteropathy [PLE]), n = 11, serum albumin concentration ≥2.5 g/dL (CIE-N). Methods: Prospective study. Lymphatic endothelial cell immunolabeling with Prox-1 and LYVE-1 performed on endoscopic biopsy samples from 24 dogs with CIE. Duodenal and ileal villous lacteal width (VLW) and proprial mucosal lacteal width (MLW) were determined for each case and analyzed for correlation with serum albumin concentration. Lacteal dilatation scores using routine H&E histopathology were assessed for correlation with immunohistochemistry (IHC)-calculated VLW and MLW. Results: Lower serum albumin concentrations were correlated with increased VLW (rho = −.4644; P =.02) and MLW (rho = −.6514; P <.001) in the ileum. Lymphatic endothelial cell IHC identified presumptive proprial mucosal lymphangiectasia in some dogs that was not recognized with routine H&E staining. Lacteal dilatation scores were correlated with VLW in duodenum (rho =.4634; P =.02) and ileum (rho =.5292; P =.008), but did not correlate with MLW. Conclusions and Clinical Importance: Lymphatic endothelial cell immunolabeling identified presumptive proprial mucosal lymphangiectasia in CIE dogs, particularly in the ileum of hypoalbuminemic dogs. Routine evaluation of villous lacteals likely underestimates abnormalities of the lymphatic vasculature in dogs with CIE.
URI: http://hdl.handle.net/10553/73103
ISSN: 0891-6640
DOI: 10.1111/jvim.15545
Fuente: Journal of Veterinary Internal Medicine [ISSN 0891-6640], v. 33(4), p. 1669-1676
Colección:Artículos
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