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Title: Microwave assisted extraction for the determination of antineoplastic compounds in marine fish
Authors: Santana Viera, Sergio 
Marzullo, Luca
Torres Padrón, María Esther 
Del Bubba, Massimo
Sosa Ferrera, María Zoraida 
Santana Rodríguez, José Juan 
UNESCO Clasification: 2301 química analítica
330811 Control de la contaminación del agua
Keywords: Antineoplastic compounds
Fish tissue
Solid samples
Microwave assisted extraction
Liquid chromatography, et al
Issue Date: 2019
Journal: Journal of Food Composition and Analysis 
Abstract: Presence of antineoplastic compounds in the marine environment could cause adverse effects even at very low concentrations. The bulk of the produced literature at present has focused on the detection of these compounds in wastewater samples, while only a few papers tackled the more complex solid samples and until now, to the best of our knowledge, none has investigated marine organisms. This work presents the development, optimisation and application of an analytical method for the extraction and determination of the four antineoplastic compounds; cyclophosphamide, vincristine, vinblastine and tamoxifen, from fish tissues by microwave assisted extraction and ultra-high-performance liquid chromatography coupled to tandem mass spectrometry. Under optimal conditions, recoveries ranged between 60% and 120%, while intra- and inter-day precision were in the 5.0–19.4% and 12.0–20.4% ranges, respectively. Method detection and quantification limits were in the ranges 0.8–1.3 and 2.7-4.3 ng·g−1 dry weight for tamoxifen and cyclophosphamide, and 46.3–536 and 154-1787 ng·g−1 dry weight for vinblastine and vincristine, respectively. The developed analytical method was successfully applied for the extraction and determination of target compounds from muscle and liver tissues belonging to three different fish species. The results did not highlight any contamination by target antineoplastic molecules in the investigated fish tissues.
ISSN: 0889-1575
DOI: 10.1016/j.jfca.2019.103241
Source: Journal of Food Composition and Analysis [ISSN 0889-1575], v. 82, 103241
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