Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/53717
Title: Simultaneous detection of mixed 'Candidatus Phytoplasma asteris' and 'Ca. Liberibacter solanacearum' infection in carrot
Authors: Satta, Eleonora
Ramirez, Ana Sofia 
Paltrinieri, Samanta
Contaldo, Nicoletta
Benito, Purificacion
Poveda, Jose Bismark 
Bertaccini, Assunta
Keywords: Zebra Chip Disease
1St Report
Hemiptera Triozidae
Potato
Association, et al
Issue Date: 2016
Publisher: 0031-9465
Journal: Phytopathologia Mediterranea 
Abstract: Symptoms of shoot and root malformation were observed in carrot plants in fields located in the North of Gran Canaria Island (Spain), during surveys carried out in spring 2015 and 2016. Total DNA extraction from the leaves of symptomatic plants was performed and PCR assays were carried out to detect the agents possibly associated to the observed symptoms. While 'Candidatus Liberibacter solanacearum' was detected in the majority of tested symptomatic samples, phytoplasmas belonging to the ribosomal group 16SrI were detected only in two samples. Phylogenetic analyses and sequencing, together with virtual RFLP, confirmed that 'Ca. L. solanacearum' strains detected in carrot samples belong to haplotype D.
URI: http://hdl.handle.net/10553/53717
ISSN: 0031-9465
DOI: 10.14601/Phytopathol_Mediterr-18683
Source: Phytopathologia Mediterranea[ISSN 0031-9465],v. 55 (3), p. 401-409
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