Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/52438
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dc.contributor.authorKalmar, Tiboren_US
dc.contributor.authorLim, Cheaen_US
dc.contributor.authorHayward, Penelopeen_US
dc.contributor.authorMuñoz-Descalzo, Silviaen_US
dc.contributor.authorNichols, Jenniferen_US
dc.contributor.authorGarcia-Ojalvo, Jordien_US
dc.contributor.authorMartinez Arias, Alfonsoen_US
dc.date.accessioned2018-11-25T20:20:08Z-
dc.date.available2018-11-25T20:20:08Z-
dc.date.issued2009en_US
dc.identifier.issn1544-9173en_US
dc.identifier.urihttp://hdl.handle.net/10553/52438-
dc.description.abstractThere is evidence that pluripotency of mouse embryonic stem (ES) cells is associated with the activity of a network of transcription factors with Sox2, Oct4, and Nanog at the core. Using fluorescent reporters for the expression of Nanog, we observed that a population of ES cells is best described by a dynamic distribution of Nanog expression characterized by two peaks defined by high (HN) and low (LN) Nanog expression. Typically, the LN state is 5%–20% of the total population, depending on the culture conditions. Modelling of the activity of Nanog reveals that a simple network of Oct4/Sox2 and Nanog activity can account for the observed distribution and its properties as long as the transcriptional activity is tuned by transcriptional noise. The model also predicts that the LN state is unstable, something that is born out experimentally. While in this state, cells can differentiate. We suggest that transcriptional fluctuations in Nanog expression are an essential element of the pluripotent state and that the function of Sox2, Oct4, and Nanog is to act as a network that promotes and maintains transcriptional noise to interfere with the differentiation signals.en_US
dc.languageengen_US
dc.relation.ispartofPLoS Biologyen_US
dc.sourcePLoS Biology[ISSN 1544-9173],v. 7 (7) (e1000149) (Julio 2009)en_US
dc.subject32 Ciencias médicasen_US
dc.subject320102 Genética clínicaen_US
dc.subject.otherPluripotencyen_US
dc.subject.otherCell differentiationen_US
dc.subject.otherGene expressionen_US
dc.subject.otherGenetic networksen_US
dc.subject.otherPopulation dynamicsen_US
dc.subject.otherTranscription factorsen_US
dc.subject.otherYellow fluorescent proteinen_US
dc.subject.otherStem cellsen_US
dc.titleRegulated fluctuations in Nanog expression mediate cell fate decisions in embryonic stem cellsen_US
dc.typeinfo:eu-repo/semantics/articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1371/journal.pbio.1000149en_US
dc.identifier.scopus68049139696-
dc.contributor.authorscopusid57205722784-
dc.contributor.authorscopusid34881904200-
dc.contributor.authorscopusid10142112100-
dc.contributor.authorscopusid9235908900-
dc.contributor.authorscopusid7201486017-
dc.contributor.authorscopusid55931225400-
dc.contributor.authorscopusid57204246803-
dc.identifier.issuee1000149-
dc.relation.volume7en_US
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.description.numberofpages16en_US
dc.utils.revisionen_US
dc.date.coverdateJulio 2009en_US
dc.identifier.ulpgcen_US
dc.contributor.buulpgcBU-MEDen_US
dc.description.scieSCIE-
item.fulltextCon texto completo-
item.grantfulltextopen-
crisitem.author.deptGIR IUIBS: Diabetes y endocrinología aplicada-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Morfología-
crisitem.author.orcid0000-0003-0939-7721-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.fullNameMuñoz Descalzo, Silvia-
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