Please use this identifier to cite or link to this item:
Title: Flow cytometric determination of lipid content in a marine dinoflagellate, Crypthecodinium cohnii
Authors: De La Jara Valido,Adelina 
Mendoza, H.
Martel, Antera 
Molina, Cristina
Nordströn, Laurette
De La Rosa Medina, Vladimir 
Díaz, Ricardo
UNESCO Clasification: 241707 Algología (ficología)
Keywords: Crypthecodinium cohnii
Flow cytometry
Lipid content
Neutral lipids, et al
Issue Date: 2003
Publisher: 0921-8971
Journal: Journal of Applied Phycology 
Abstract: Since flow cytometry allows rapid,simultaneous and quantitative measurementsrelated to cell morphology andphysiologicy, the lipid-specificfluorescent dye, Nile Red, was employed forthe in vivo lipid quantification of Crypthecodinium cohnii, a heterotrophicmarine dinoflagellate rich inpolyunsaturated long chain fatty acids. Thefluorescence signal was linearly correlatedwith the neutral and polar lipid content asdetermined by gravimetric techniques. Asignificant correlation of NR signal wasalso observed between the polar to neutrallipid ratio and docohexaenoic acid percell. The results demonstrate a method forrapid lipid quantification that can be usedin the selection, isolation and culturecontrol of C. cohnii clones with highlipid and DHA content.
ISSN: 0921-8971
DOI: 10.1023/A:1026007902078
Source: Journal of Applied Phycology [ISSN 0921-8971], v. 15, p. 433-438
Appears in Collections:Artículos
Show full item record

Google ScholarTM




Export metadata

Items in accedaCRIS are protected by copyright, with all rights reserved, unless otherwise indicated.