Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/51243
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dc.contributor.authorCarmona, J. U.
dc.contributor.authorRíos, E.
dc.contributor.authorVilar, J. M.
dc.contributor.authorGiraldo, C. E.
dc.contributor.authorLópez, C.
dc.date.accessioned2018-11-24T22:47:17Z-
dc.date.available2018-11-24T22:47:17Z-
dc.date.issued2014
dc.identifier.issn0301-732X
dc.identifier.urihttp://hdl.handle.net/10553/51243-
dc.description.abstractThe aim of this study was to compare plasma rico en plaquetas (PRP) obtained from blood of cows using sodium citrate (SC) or citrate, citric acid, dextrose-solution A (ACD-A) anticoagulants regarding the content of platelets and leukocytes (WBC), and the degree of platelet activation in whole blood (WB) and (PRP). Blood samples were collected from 15 clinically healthy Holstein cows in tubes with SC and ACD-A. Samples were processed by a double centrifugation tube method for producing PRP. PRP and WB were analysed for hemogram and automated determination of mean platelet volume (MPV) and platelet distribution width volume (PDW). Platelet counts differed (P < 0.0001) between WB and PRP for each separate anticoagulant. The concentration of platelets in PRP was 2,3 and 2,5 times for SC and ACD-A, respectively, compared to platelet counts in WB for both anticoagulants. PRP platelet concentration did not differ between both anticoagulants. Values for MPV and PDW were similar in PRP obtained after centrifugations regardless of the anticoagulant used. Leukocyte counts were higher (P < 0.001), particularly lymphocytes, in PRP obtained with SC in comparison with ACD-A. In conclusion, results obtained allowed to establish that there are no differences for obtaining bovine PRP, using either SC or ACD-A as anticoagulants. The results suggest platelet conservation homogeneous and structurally stable in bovine PRP, especially when sodium citrate was used.
dc.publisher0301-732X
dc.relation.ispartofArchivos de Medicina Veterinaria
dc.sourceArchivos de Medicina Veterinaria[ISSN 0301-732X],v. 46, p. 375-380
dc.subject.otherTube Method
dc.subject.otherSurgery
dc.subject.otherQuality
dc.subject.otherPrf
dc.subject.otherGel
dc.titleEffect of two anticoagulants on the cell count and platelet activation parameters from bovine platelet rich plasma
dc.typeinfo:eu-repo/semantics/Articlees
dc.typeArticlees
dc.identifier.scopus84921346313
dc.identifier.isi000348180700005
dc.contributor.authorscopusid29067507900
dc.contributor.authorscopusid56487221100
dc.contributor.authorscopusid7005533720
dc.contributor.authorscopusid25521769000
dc.contributor.authorscopusid35196490500
dc.description.lastpage380
dc.description.firstpage375
dc.relation.volume46
dc.type2Artículoes
dc.contributor.daisngid30381678
dc.contributor.daisngid21948088
dc.contributor.daisngid913655
dc.contributor.daisngid609709
dc.contributor.daisngid1391720
dc.contributor.wosstandardWOS:Carmona, JU
dc.contributor.wosstandardWOS:Rios, E
dc.contributor.wosstandardWOS:Vilar, JM
dc.contributor.wosstandardWOS:Giraldo, CE
dc.contributor.wosstandardWOS:Lopez, C
dc.date.coverdateEnero 2014
dc.identifier.ulpgces
dc.description.sjr0,196
dc.description.jcr0,306
dc.description.sjrqQ3
dc.description.jcrqQ4
item.grantfulltextnone-
item.fulltextSin texto completo-
crisitem.author.deptGIR IUIBS: Medicina Veterinaria e Investigación Terapéutica-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.orcid0000-0002-2060-2274-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.fullNameVilar Guereño, José Manuel-
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