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Title: A comparative study of the glutamate dehydrogenase activity in several species of marine phytoplankton
Authors: Ahmed, S. I.
Kenner, R. A.
Packard, T. T. 
UNESCO Clasification: 241705 Biología marina
2302 Bioquímica
Issue Date: 1976
Publisher: 0025-3162
Journal: Marine Biology 
Abstract: Kinetic studies on the NADP-linked glutamate dehydrogenase (GDH) activity in 8 species of marine phytoplankton have been carried out. The pH optima, linearity of reactions, apparent energies of activation, and apparent K m values for NADPH, NH 4 >+ and α-ketoglutarate were determined. Based on these data, an accurate, sensitive enzyme assay procedure is presented which was successfully tested on shipboard during a recent cruise in an upwelling region. Preliminary studies on inhibition and activation of the NADP-linked GDH activity have also been carried out. In addition to NADP-linked GDH activity, NAD-linked GDH activity was discovered in certain species of phytoplankton. The possible physiological roles of NAD-and NADP-linked GDH enzymes are discussed.
ISSN: 0025-3162
DOI: 10.1007/BF00395598
Source: Marine Biology [ISSN 0025-3162], v. 39, p. 93-101
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