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Title: CO2 production rate predicted from isocitrate dehydrogenase activity, intracellular substrate concentrations and kinetic constants in the marine bacterium Pseudomonas nautica
Authors: Roy, S. O.
Packard, T. 
UNESCO Clasification: 251001 Oceanografía biológica
Keywords: Enzyme
Kinetic Model
Nutrient Source
Substrate Concentration
Issue Date: 2001
Publisher: 0025-3162
Journal: Marine Biology 
Abstract: CO2 production rates in all growth phases of the marine bacterium Pseudomonas nautica have been modeled using bisubstrate enzyme kinetics, isocitrate dehydrogenase activity and measurements of intracellular isocitrate and NADP+. The mathematical model has been adapted from a previous publication and modified by incorporating newly measured kinetic constants, and new experimentally determined algorithms for intracellular isocitrate and NADP+. The model can predict the rate of CO2 production for up to 14 days and successfully describes the transition from exponential growth to senescence that occurs within the first 24 h as the organic nutrient source is depleted. A single substrate kinetic model of the rate of CO2 production was also tested, but was found to be less descriptive of the observations than the bisubstrate model. Future improvement of the model should account for variations of the physiological culture conditions, a revision of the isocitrate algorithm, and the addition of malic enzyme and phophoenolpyruvate carboxykinase terms.
ISSN: 0025-3162
DOI: 10.1007/s002270100550
Source: Marine Biology [ISSN 0025-3162], v. 138, p. 1251-1258
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