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http://hdl.handle.net/10553/48449
Title: | An helicoidal structure surrounding the cilium axoneme: Visualization by the monoclonal antibody CC-248 | Authors: | Bautista-Harris, Guillermina Klotz, Catherine Bordes, Nicole Sandoz, Daniel |
UNESCO Clasification: | 32 Ciencias médicas 2407 Biología celular |
Keywords: | Cilium axoneme Antibody CC-248 |
Issue Date: | 1991 | Journal: | Biology of the Cell | Abstract: | Monoclonal antibody CC-248 labels cilia differentially on Triton X-100 permeabilized ciliated epithelium of quail oviduct by indirect immunofluorescence. On isolated ciliated cells, a punctuated staining is seen at the distal region over the bend of cilia. Electron micrographs of immunoperoxidase and immunogold techniques showed that the punctuated fluorescence corresponds to a helical disposition of CC-248 antigenic sites. This labeling was arranged on the axonemal distal region either as a simple or a double helix externally disposed around the nine microtubular doublets. These results suggest the existence of a detergent insoluble structure in the ciliary matrix that might concern the ciliary skeleton, probably acting as an elastic recoil that keeps the structural integrity of the axoneme during bending. The cross-reactivity of CC-248 MAb with the intermediate filament cytoskeleton of ciliated and smooth muscle cells indicates that this structure might be related to the intermediate filament family. | URI: | http://hdl.handle.net/10553/48449 | ISSN: | 0248-4900 | DOI: | 10.1016/0248-4900(91)90065-U | Source: | Biology of the Cell[ISSN 0248-4900],v. 71, p. 191-200 |
Appears in Collections: | Artículos |
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