Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/47909
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dc.contributor.authorNegrín-Báez, Davinia
dc.contributor.authorNavarro, Ana
dc.contributor.authorLee-Montero, Ivonne
dc.contributor.authorAfonso, Juan M.
dc.contributor.authorSánchez, Juan J.
dc.contributor.authorElalfy, Islam S.
dc.contributor.authorManchado, Manuel
dc.contributor.authorSánchez, José A.
dc.contributor.authorGarcía-Celdrán, Marta
dc.contributor.authorZamorano, María J.
dc.contributor.otherManchado, Manuel
dc.contributor.otherZamorano, Maria Jesus
dc.date.accessioned2018-11-23T17:25:13Z-
dc.date.available2018-11-23T17:25:13Z-
dc.date.issued2015
dc.identifier.issn1355-557X
dc.identifier.urihttp://hdl.handle.net/10553/47909-
dc.description.abstract© 2014 John Wiley & Sons Ltd.The growth and consolidation of the gilthead seabream (Sparus aurata) industry require improvement based on permanent and cumulative aspects, such as those derived from genetic breeding programmes. Marker Assisted Selection (MAS) by Quantitative Trait Loci (QTL) can be usefully implemented with the appropriate tools. In this study, 138 microsatellite markers from the genetic map of gilthead seabream were redesigned to be amplified under the same PCR conditions. A final set of 13 multiplex PCRs (named ReMsa) with 106 of these markers was developed to cover 100% of the linkage groups. These effective multiplex PCRs enable to optimize QTL searching with a critical reduction in costs and errors. Results showed that the mean value of the number of alleles for 106 markers was 6.9. The mean observed heterozygosity ranged from 0.53 to 0.86, and 74.5% of markers were highly informative according to their polymorphic information content. The correct inheritance and segregation of alleles of each locus was confirmed after genotyping 62 individuals of a full-sib family by these multiplex PCRs. In addition, genetic features of the 20 microsatellite markers that worked correctly but were not included in any multiplex PCR are also reported to provide geneticists with the possibility of including them in more comprehensive screening studies.
dc.publisher1355-557X
dc.relation.ispartofAquaculture Research
dc.sourceAquaculture Research[ISSN 1355-557X],v. 46, p. 45-58
dc.titleA set of 13 multiplex PCRs of specific microsatellite markers as a tool for QTL detection in gilthead seabream (Sparus aurata L.)
dc.typeinfo:eu-repo/semantics/Articlees
dc.typeArticlees
dc.identifier.doi10.1111/are.12378
dc.identifier.scopus84923540510
dc.identifier.isi000350467500006
dcterms.isPartOfAquaculture Research
dcterms.sourceAquaculture Research[ISSN 1355-557X],v. 46, p. 45-58
dc.contributor.authorscopusid55644309600
dc.contributor.authorscopusid13612121000
dc.contributor.authorscopusid57212839113
dc.contributor.authorscopusid55644147300
dc.contributor.authorscopusid57201126472
dc.contributor.authorscopusid7403998988
dc.contributor.authorscopusid56012368300
dc.contributor.authorscopusid6603277540
dc.contributor.authorscopusid55701687700
dc.contributor.authorscopusid55224003000
dc.contributor.authorscopusid6701451831
dc.description.lastpage58
dc.description.firstpage45
dc.relation.volume46
dc.type2Artículoes
dc.identifier.wosWOS:000350467500006
dc.contributor.daisngid4127459
dc.contributor.daisngid2095438
dc.contributor.daisngid5976729
dc.contributor.daisngid1136306
dc.contributor.daisngid7060378
dc.contributor.daisngid23725901
dc.contributor.daisngid285240
dc.contributor.daisngid560195
dc.contributor.daisngid4090047
dc.contributor.daisngid1009633
dc.identifier.investigatorRIDM-8379-2015
dc.identifier.investigatorRIDM-5052-2018
dc.date.coverdateEnero 2015
dc.identifier.ulpgces
dc.description.sjr0,79
dc.description.jcr1,606
dc.description.sjrqQ2
dc.description.jcrqQ2
dc.description.scieSCIE
item.grantfulltextnone-
item.fulltextSin texto completo-
crisitem.author.deptGIR Grupo de Investigación en Acuicultura-
crisitem.author.deptIU de Investigación en Acuicultura Sostenible y Ec-
crisitem.author.deptDepartamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.deptGIR Grupo de Investigación en Acuicultura-
crisitem.author.deptIU de Investigación en Acuicultura Sostenible y Ec-
crisitem.author.deptDepartamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.orcid0000-0003-1569-9152-
crisitem.author.parentorgIU de Investigación en Acuicultura Sostenible y Ec-
crisitem.author.parentorgIU de Investigación en Acuicultura Sostenible y Ec-
crisitem.author.fullNameAfonso López, Juan Manuel-
crisitem.author.fullNameZamorano Serrano, María Jesús-
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