|Title:||The invitro immunomodulatory effect of extracellular products (ECPs) of Vagococcus fluvialis L21 on European sea bass (Dicentrarchus labrax) leucocytes||Authors:||Román, L.
El Aamri, F.
Real Valcárcel, Fernando
Innate Immune Parameters
Aeromonas-Salmonicida, et al
|Issue Date:||2015||Publisher:||1050-4648||Journal:||Fish and Shellfish Immunology||Abstract:||The immune associated genes, interleukin-1 beta (IL-1 beta), interleukin-6 (IL-6), interleukin 10 (IL-10), tumor necrosis factor-alpha (TNF-alpha), ciclo-oxigenase-2 (COX-2), and Mx gene were studied by real-time PCR in head-kidney leucocytes of sea bass after incubation with the extracellular products (ECPs) of the probiotic strain Vagococcus fluvialis L21 and polyinosinic:polycytidylic acid (POLY 1:C), at different times (T1.5, T6, T12, T24, T48 and T72). In general, we can observe how pro-inflammatory cytokines IL-1 beta, TNF-alpha, IL-6 and COX-2 studied displayed a strong peak after stimulation with 1.5 h of ECPs of V. fluvialis 121, significant differences (P < 0.05) exist with other periods and with the POLY I: C at the same time. Similarly to the case of IL-10 also produced a statistically significant (P < 0.05) peak of expression on leukocytes that were stimulated with the ECPs of V. fluvialis L21. In the case of Mx gene expression, we note that in almost all sampling times there is an up-regulation of the Mx gene in leucocytes incubated with ECPs and POLY I:C compared to the control and Mx expression was higher in leucocytes that were stimulated with the ECPs of V. fluvialis for all times, except in T24. With these results we can consider that the ECPs of V. fluvialis L21 have a great power of stimulating the in vitro expression of immune-related genes and may even be useful as adjuvants for vaccine in aquaculture. (C) 2014 Elsevier Ltd. All rights reserved.||URI:||http://hdl.handle.net/10553/47226||ISSN:||1050-4648||DOI:||10.1016/j.fsi.2014.11.037||Source:||Fish and Shellfish Immunology[ISSN 1050-4648],v. 42, p. 517-521|
|Appears in Collections:||Artículos|
checked on May 15, 2022
WEB OF SCIENCETM
checked on May 23, 2021
checked on Jan 8, 2022
Items in accedaCRIS are protected by copyright, with all rights reserved, unless otherwise indicated.