Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/47190
Title: Micellar enhanced spectrofluorometric determination of chlorophyll a and chlorophyll b in fresh waters
Authors: Santana, Jose J. 
Gunshefski, Mary Ann
Winefordner, James D.
UNESCO Clasification: 2301 química analítica
Keywords: Micellar media
Spectrofluorometry
Chlorophyll a
Chlorophyll b
Fresh waters
Issue Date: 1992
Publisher: 0039-9140
Journal: Talanta 
Abstract: The enhancement of the fluorescence of chlorophyll a and chlorophyll b in a non-ionic micellar media is used for the establishment of a new analytical method for the determination of these pigments. After optimization of the variables, including concentration of surfactant, pH, temperature and percentage of organic solvent, the analytical figures of merit are determined. High recovery percentages are found when the method is applied to the simultaneous determination of chlorophyll a and b in synthetic mixtures. Finally, the method is used for the determination of both chlorophylls in local fresh waters, finding excellent agreement with the results obtained by an HPLC/fluorescence method.
URI: http://hdl.handle.net/10553/47190
ISSN: 0039-9140
DOI: 10.1016/0039-9140(92)80019-A
Source: Talanta [ISSN 0039-9140], v. 39, p. 195-200
Appears in Collections:Artículos
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