Estudio Histoquímico y Morfométrico de la Población Fibrilar del Müsculo Diafragma. Parte II: Vaca y Cerdo

Abstract

Histochemical and Morphometric Study of the Fibrilar Population in Diaphragm Muscle. Part II: Ox and Pig M‐ATPase techniques, at different pH levels prior to incubation, as well as metabolic procedures (α‐MGPDH and NADH‐TR), were used to determine the fibre‐type content of the lumbar and costal portions in the bovine and porcine diaphragm. The fibre samples were exposed to image analysis in order to evaluate both the morphometrical and statistical values. On the basis of tintorial reactions, the fibre types IA, IB and IIC were found in the lumbar portion of the bovine diaphragm; in the costal portion of the same diaphragm, IA, IB, IIA and IIC fibre types were observed. With regard to the porcine diaphragm, the I, IIA, IIB, IIC fibre types were present in both portions of the muscle. Further morphometrical and statistical data concerning all the fibre types are obtained and discussed.