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Title: Results of the GEP-ISFG collaborative study on an X-STR Decaplex
Authors: Gusmão, L.
Alves, C.
Sánchez-Diz, P.
Zarrabeitia, M. T.
Abovich, M. A.
Aragón, I.
Arce, B.
Arrieta, G.
Arroyo, E.
Atmetlla, I.
Baeza, C.
Bobillo, M. C.
Cainé, L.
Campos, R.
Caraballo, L.
Carvalho, E.
Carvalho, M.
Cicarelli, R. M.B.
Comas, D.
Corach, D.
Espinoza, M.
Espinheira, M. R.
Rendo, F.
García, O.
Gomes, I.
González, A. 
Hernández Ballester, Antonio 
Hidalgo, M.
Lozano, P.
Malaghini, M.
Manzanares, D.
Martínez, B.
Martins, J. A.
Maxzud, K.
Miguel, I.
Modesti, N.
Montesino, M.
Ortiz, R.
Pestano, J. J. 
Pinheiro, M. F.
Prieto, L.
Raimondi, E.
Riancho, J. A.
Rodríguez, M. B.
Salgado, I.
Salgueiro, N.
Sánchez, J. J.
Silva, S.
Toscanini, U.
Vidales, C.
Silva, C. V.
Villalobos, M. C.
Vullo, C.
Yurrebaso, I.
Zubillaga, A. I.
Carracedo, A.
Amorim, A.
UNESCO Clasification: 32 Ciencias médicas
320102 Genética clínica
Keywords: X-STRs
Multiplex PCR
Issue Date: 2008
Journal: Forensic Science International: Genetics Supplement Series 
Abstract: A collaborative work was carried out by the Spanish and Portuguese ISFG Working Group with a PCR multiplex for X chromosome STRs. Markers were selected among those described as polymorphic in humans and that have been used by some laboratories in forensics. Primers and various technical methods were investigated with the aim of optimizing a multiplex for the 10 selected X-STRs. Primer mix stock solutions were sent to the laboratories that were asked to analyse two female bloodstains, taking as reference the genetic profiles from 9947A, 9948 and NA3657 samples. In this work, we report the results obtained by 30 GEP-ISFG laboratories, using this Decaplex, as well as alternative technical conditions that also produced good results.
ISSN: 1875-1768
DOI: 10.1016/j.fsigss.2007.10.012
Source: Forensic Science International: Genetics Supplement Series[ISSN 1875-1768],v. 1, p. 677-679
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