Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/46967
DC FieldValueLanguage
dc.contributor.authorFregel, R.en_US
dc.contributor.authorAlmeida, M.en_US
dc.contributor.authorBetancor, E.en_US
dc.contributor.authorSuárez, N. M.en_US
dc.contributor.authorPestano, J.en_US
dc.date.accessioned2018-11-23T09:47:26Z-
dc.date.available2018-11-23T09:47:26Z-
dc.date.issued2011en_US
dc.identifier.issn1875-1768en_US
dc.identifier.otherWoS-
dc.identifier.otherScopus-
dc.identifier.urihttp://hdl.handle.net/10553/46967-
dc.description.abstractDNA quantification is a prerequisite for both low copy number (LCN) forensic analysis and ancient DNA (aDNA) studies. Moreover, if nuclear quantification is focused on the amelogenin locus, it also allows sex determination. Some of the problems of these techniques are allelic drop-out phenomenon in amelogenin locus and mitochondrial DNA (mtDNA) quantification biases, due to human intraspecific variation affecting the annealing of primers and/or probes.The method presented here combines two multiplex TaqMan (R) real-time PCR (qPCR) for nuclear and mtDNA quantification in degraded or limited samples. Nuclear DNA detection is based on the independent amplification of X and Y chromosome specific fragments in the amelogenin locus and an internal PCR control (IPC) to recognize inhibition problems. The small length of the fragments (71 bp) favors the quantification of severely degraded DNA, whereas the use of two distinct primer sets for X and Y chromosome amplification is directed to reduce allelic drop-out in LCN analysis. MtDNA quantification is based on the amplification of three PCR fragments located in the mtDNA 16S region. Two of them are amplified with human specific conservative primers and probes, which allows a world-wide application of this technique. Moreover, their length difference (167 and 314 bp respectively), provides information about the DNA degradation level. In order to also recognize non-human DNA an interspecific mtDNA fragment (187 bp) was also designed.en_US
dc.languageengen_US
dc.relation.ispartofForensic Science International: Genetics Supplement Seriesen_US
dc.sourceForensic Science International Genetics Supplement Series [ISSN 1875-1768], v. 3 (1), p. e303-e304, (Diciembre 2011)en_US
dc.subject2409 Genéticaen_US
dc.subject.otherReal-Time Pcren_US
dc.subject.otherAncient Dnaen_US
dc.subject.otherLcn Sampleen_US
dc.subject.otherDna Quantificationen_US
dc.subject.otherNuclear Dnaen_US
dc.subject.otherMitochondrial Dnaen_US
dc.titleReliable nuclear and mitochondrial DNA quantification for low copy number and degraded forensic samplesen_US
dc.typeinfo:eu-repo/semantics/articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.fsigss.2011.09.014en_US
dc.identifier.scopus2-s2.0-82455162371-
dc.identifier.scopus82455162371-
dc.identifier.isi000420449400152-
dc.contributor.authorscopusid8895291800-
dc.contributor.authorscopusid26533827100-
dc.contributor.authorscopusid23979618400-
dc.contributor.authorscopusid7003499062-
dc.contributor.authorscopusid56149756400-
dc.identifier.eissn1875-175X-
dc.description.lastpageE304en_US
dc.identifier.issue1-
dc.description.firstpageE303en_US
dc.relation.volume3en_US
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.contributor.daisngid1161749-
dc.contributor.daisngid5446169-
dc.contributor.daisngid2080005-
dc.contributor.daisngid738179-
dc.contributor.daisngid865329-
dc.description.numberofpages2en_US
dc.utils.revisionen_US
dc.contributor.wosstandardWOS:Fregel, R-
dc.contributor.wosstandardWOS:Almeida, M-
dc.contributor.wosstandardWOS:Betancor, E-
dc.contributor.wosstandardWOS:Suarez, NM-
dc.contributor.wosstandardWOS:Pestano, J-
dc.date.coverdateDiciembre 2011en_US
dc.identifier.ulpgces
dc.description.sjr0,374
dc.description.sjrqQ3
dc.description.esciESCI
item.fulltextSin texto completo-
item.grantfulltextnone-
crisitem.author.deptMedio Ambiente y Salud-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptMedio Ambiente y Salud-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptBioquímica y Biología Molecular, Fisiología, Genética e Inmunología.-
crisitem.author.orcid0000-0002-9958-9382-
crisitem.author.orcid0000-0001-6454-4785-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.fullNameBetancor Hernández, Eva Del Carmen-
crisitem.author.fullNamePestano Brito, José Juan-
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