Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/46900
Title: Subcellular immuno‐localization, amino acid composition and partial amino acid sequences of α‐l,4‐glucan phosphorylase of Gracilaria spp (Rhodophyta)
Authors: Yu, S.
Gómez‐Pinchetti, J. ‐L 
Garcia-Blairsy Reina, Guillermo 
Ek, B.
Pedersén, M.
UNESCO Clasification: 241719 Fisiología vegetal
241705 Biología marina
Keywords: Gracilariales
α‐1,4‐glucan phosphorylase
Immuno‐gold localization
Rhodophyta
Starch metabolism
Issue Date: 1993
Publisher: 0031-9317
Journal: Physiologia Plantarum 
Abstract: Antibodies have been raised against an α‐l,4‐glucan phosphorylase (EC 2. 4. 1. 1) purified from the red alga Gracilaria chilensis. Localization of α‐l,4‐glucan phosphorylase in thin sections of G. chilensis and G. tenuistipitata was performed using immuno‐gold labelling and transmission electron microscopy. The enzyme was localized in the cytosol and around the cytosolic starch granules of the algal cells. The labelling was not associated with the chloroplast or the cell wall. Amino acid composition of the red algal phosphorylase was quite similar to that of potato tuber and rabbit muscle phosphorylases. Partial amino acid sequences showed 48, 54 and 65% homology with the rabbit, potato and Escherichia coli enzymes, respectively.
URI: http://hdl.handle.net/10553/46900
ISSN: 0031-9317
DOI: 10.1111/j.1399-3054.1993.tb01780.x
Source: Physiologia Plantarum [ISSN 0031-9317], v. 89, p. 11-20
Appears in Collections:Artículos
Show full item record

SCOPUSTM   
Citations

4
checked on Jun 13, 2021

WEB OF SCIENCETM
Citations

4
checked on Jun 13, 2021

Page view(s)

37
checked on Jun 14, 2021

Google ScholarTM

Check

Altmetric


Share



Export metadata



Items in accedaCRIS are protected by copyright, with all rights reserved, unless otherwise indicated.