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http://hdl.handle.net/10553/44898
Título: | Quantification of cyclamate and cyclohexylamine in urine samples using high-performance liquid chromatography with trinitrobenzenesulfonic acid pre-column derivatization | Autores/as: | Casals, Isidre Reixach, Maria Amat, Judith Fuentes, Margarita Serra-Majem, Lluís |
Clasificación UNESCO: | 32 Ciencias médicas 3212 Salud pública 230103 Análisis cromatográfico |
Palabras clave: | Cyclamate Cyclohexylamine Trinitrobenzenesulfonic acid Derivatization, LC |
Fecha de publicación: | 1996 | Publicación seriada: | Journal of Chromatography A | Resumen: | An HPLC isocratic method with pre-column derivatization and UV detection for the quantification of cyclamate and cyclohexylamine in urine samples is described. The method requires very little sample preparation. Free cyclohexylamine is analysed in a first run and subsequently cyclamate is analysed as cyclohexylamine, after the simple process of oxidation of the sample by means of hydrogen peroxide. Cycloheptylamine is used as internal standard. Trinitrobenzenesulfonic acid (TNBS) appears to be a good reagent for the pre-column derivatization. The time per run is 15 min; the coefficients of variation of the assays range from 1.1 to 5.5%; the limits of detection are 0.09 and 0.11 ppm for cyclohexylamine and cyclamate anion, respectively. The system described has always performed efficiently, with a high degree of stability, in daily routine work. | URI: | http://hdl.handle.net/10553/44898 | ISSN: | 0021-9673 | DOI: | 10.1016/0021-9673(96)00529-8 | Fuente: | Journal of Chromatography A [ISSN 0021-9673], v. 750, p. 397-402 |
Colección: | Artículos |
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