Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/44451
Título: Single-cell fura-2 microfluorometry reveals different purinoceptor subtypes coupled to Ca2+ influx and intracellular Ca2+ release in bovine adrenal chromaffin and endothelial cells
Autores/as: Castro López-Tarruella, Enrique 
Tomé, Angelo R.
Miras-Portugal, Maria Teresa
Rosário, Luís M.
Clasificación UNESCO: 32 Ciencias médicas
Palabras clave: Purinergic receptors
Adenosine triphosphate
Cytosolic calcium
Diadenosine polyphosphates
Cellular heterogeneity, et al.
Fecha de publicación: 1994
Editor/a: 0031-6768
Publicación seriada: Pflugers Archiv European Journal of Physiology 
Resumen: ATP and adenosine(5′)tetraphospho(5′)adenosine (Ap4A), released from adrenal chromaffin cells, are potent stimulators of endothelial cell function. Using single-cell fura-2 fluorescence recording techniques to measure free cytosolic Ca2+ concentration ([Ca2+]i), we have investigated the role of purinoceptor subtypes in the activation of cocultured chromaffin and endothelial cells. ATP evoked concentration-dependent [Ca2+]i rises (EC50=3.8 μM) in a subpopulation of chromaffin cells. Both ATP-sensitive and -insensitive cells were potently activated by nicotine, bradykinin and muscarine. Reducing extracellular free Ca2+ concentration to around 100 nM suppressed the [Ca2+]i transient evoked by ATP but not the [Ca2+]i response to bradykinin. ATP-sensitive chromaffin cells were also potently stimulated by 2-methylthioadenosine triphosphate (2MeSATP; EC50= 12.5 μM) and UTP, but did not respond to either adenosine 5′-[β-thio]diphosphate (ADP[βS]), a P2Y receptor agonist, adenosine 5′-[α,β-methylene]triphosphate (pp[CH2]pA), a P2X agonist or AMP. Adrenal endothelial cells displayed concentration-dependent [Ca2+]i responses when stimulated with ATP (EC50=0.86 μM), UTP (EC50=1.6 μM) and 2MeSATP (EC50= 0.38 μM). 2MeSATP behaved as a partial agonist. Ap4A and ADP[βS] also raised the [Ca2+]i in endothelial cells, whereas AMP and pp[CH2]pA were ineffective. Lowering extracellular free Ca2+ to around 100 nM did not affect the peak ATP-evoked [Ca2+]i rise in these cells. It is concluded that different purinoceptor subtypes are heterogeneously distributed among the major cell types of the adrenal medulla. An intracellular Ca2+-releasing P2U-type purinoceptor is specifically localized to adrenal endothelial cells, while a subpopulation of chromaffin cells expresses a non-P2X, non-P2Y subtype exclusively coupled to Ca2+ influx.
URI: http://hdl.handle.net/10553/44451
ISSN: 0031-6768
DOI: 10.1007/BF00378530
Fuente: Pflügers Archiv European Journal of Physiology [ISSN 0031-6768], v. 426, p. 524-533
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