Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/44431
Título: Cerebellar astrocytes coexpress different purinoceptors: Cross-talk between several transduction mechanisms
Autores/as: Delicado, Esmerilda G.
Jiménez, Ana I.
Castro López-Tarruella, Enrique 
Miras-Portugal, Ma Teresa
Clasificación UNESCO: 32 Ciencias médicas
Palabras clave: Inhibits Adenylyl-Cyclase
Molecular-Cloning
Calcium Responses
Endothelial-Cells
Phospholipase-C, et al.
Fecha de publicación: 2001
Editor/a: 0272-4391
Publicación seriada: Drug Development Research 
Conferencia: PURINES 2000 Meeting 
Resumen: We characterized the purinergic receptors present in individual type-1 astrocytes from rat cerebellum by using single cell microfluorimetry with fura-2. We found that individual astrocytes coexpressed several P2Y purinoceptors coupled to phospholipase C activation through different G proteins. P2Y1 and P2Y2 or P2Y4 purinoceptors were responsible for the ATP, 2MeSATP, and UTP calcium responses observed in all tested cell. A subpopulation of astrocytes also coexpressed a specific pyrimidine receptor, which was responsible for UDP calcium responses recorded only from a subpopulation of astrocytes. Type-1 astrocytes also present A2B adenosine receptors positively coupled to adenylate cyclase. There was a positive interaction between A2B adenosine receptors and P2Y1 or P2Y2 or P2Y4 purinoceptors expressed in the same astrocyte. We also found that the stimulation of astrocytes with nanomolar concentrations of Ap5A produced the activation of extracellular signal regulated kinases (ERK), which could be responsible for the potentiation of ATP calcium responses elicited by the dinucleotide.
URI: http://hdl.handle.net/10553/44431
ISSN: 0272-4391
DOI: 10.1002/ddr.1105
Fuente: Drug Development Research [ISSN 0272-4391], v. 52, p. 114-121
Colección:Actas de congresos
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