Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/44431
Title: Cerebellar astrocytes coexpress different purinoceptors: Cross-talk between several transduction mechanisms
Authors: Delicado, Esmerilda G.
Jiménez, Ana I.
Castro López-Tarruella, Enrique 
Miras-Portugal, Ma Teresa
UNESCO Clasification: 32 Ciencias médicas
Keywords: Inhibits Adenylyl-Cyclase
Molecular-Cloning
Calcium Responses
Endothelial-Cells
Phospholipase-C
Glial Calcium
Atp Receptor
Activation
Adenosine
P-2Y-Purinoceptors
Issue Date: 2001
Publisher: 0272-4391
Journal: Drug Development Research 
Conference: PURINES 2000 Meeting 
Abstract: We characterized the purinergic receptors present in individual type-1 astrocytes from rat cerebellum by using single cell microfluorimetry with fura-2. We found that individual astrocytes coexpressed several P2Y purinoceptors coupled to phospholipase C activation through different G proteins. P2Y1 and P2Y2 or P2Y4 purinoceptors were responsible for the ATP, 2MeSATP, and UTP calcium responses observed in all tested cell. A subpopulation of astrocytes also coexpressed a specific pyrimidine receptor, which was responsible for UDP calcium responses recorded only from a subpopulation of astrocytes. Type-1 astrocytes also present A2B adenosine receptors positively coupled to adenylate cyclase. There was a positive interaction between A2B adenosine receptors and P2Y1 or P2Y2 or P2Y4 purinoceptors expressed in the same astrocyte. We also found that the stimulation of astrocytes with nanomolar concentrations of Ap5A produced the activation of extracellular signal regulated kinases (ERK), which could be responsible for the potentiation of ATP calcium responses elicited by the dinucleotide.
URI: http://hdl.handle.net/10553/44431
ISSN: 0272-4391
DOI: 10.1002/ddr.1105
Source: Drug Development Research [ISSN 0272-4391], v. 52, p. 114-121
Appears in Collections:Actas de congresos
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