Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/44422
Título: Efficient synaptic vesicle recycling after intense exocytosis concomitant with the accumulation of non-releasable endosomes at early developmental stages
Autores/as: Bartolomé-Martín, David
Ramírez-Franco, Jorge
Castro López-Tarruella, Enrique 
Sánchez-Prieto, José
Torres, Magdalena
Clasificación UNESCO: 32 Ciencias médicas
Palabras clave: Cerebellar granule cells
Dynasore
Endocytosis
FM1-43
Synaptic vesicle cycle
Fecha de publicación: 2012
Editor/a: 0021-9533
Publicación seriada: Journal of Cell Science 
Resumen: Following the exocytosis of neurotransmitter-containing synaptic vesicles, endocytosis is fundamental to re-establishing conditions for synaptic transmission. As there are distinct endocytotic pathways that each differ in their efficiency to generate releasable synaptic vesicles, we used the dye FM1-43 to track vesicle recycling, and to determine whether nerve terminals use multiple pathways of endocytosis. We identified two types of synaptic boutons in cultured cerebellar granule cells that were characterized by weak or strong FM1-43-unloading profiles. Decreasing the extent of exocytosis dramatically increased the proportion of synaptic boutons that exhibited strong FM1-43-unloading and dramatically reduced the number of endosome-like structures. Hence, we concluded that efficient recycling of synaptic vesicles is concomitant with the formation of non-releasable endosomes in both types of synaptic boutons, although to different extents. Furthermore, cell maturation in culture increased the proportion of synaptic boutons that were capable of an intense release response, whereas the chronic blockage of synaptic activity diminished the capacity of boutons to release dye.
URI: http://hdl.handle.net/10553/44422
ISSN: 0021-9533
DOI: 10.1242/jcs.090878
Fuente: Journal of Cell Science [ISSN 0021-9533], v. 125, p. 422-434
Colección:Artículos
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