Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/135410
Title: Unexpected Genetic Diversity of Nostocales (Cyanobacteria) Isolated from the Phyllosphere of the Laurel Forests in the Canary Islands (Spain)
Authors: Rancel-Rodríguez, Nereida M.
Sausen, Nicole
Reyes, Carolina P.
Martel Quintana, Antera 
Melkonian, Barbara
Melkonian, Michael
UNESCO Clasification: 2414 Microbiología
Keywords: Biodiversity
Clonal Culture
Epiphyllous
Heterocyte-forming cyanobacteria
rRNA sequence comparison
Issue Date: 2024
Journal: Microorganisms 
Abstract: A total of 96 strains of Nostocales (Cyanobacteria) were established from the phyllosphere of the laurel forests in the Canary Islands (Spain) and the Azores (Portugal) using enrichment media lacking combined nitrogen. The strains were characterized by light microscopy and SSU rRNA gene comparisons. Morphologically, most strains belonged to two different morphotypes, termed “Nostoc-type” and “Tolypothrix-type”. Molecular phylogenetic analysis of 527 SSU rRNA gene sequences of cyanobacteria (95 sequences established during this study plus 392 sequences from Nostocales and 40 sequences from non-heterocyte-forming cyanobacteria retrieved from the databases) revealed that none of the SSU rRNA gene sequences from the phyllosphere of the laurel forests was identical to a database sequence. In addition, the genetic diversity of the isolated strains was high, with 42 different genotypes (44% of the sequences) recognized. Among the new genotypes were also terrestrial members of the genus Nodularia as well as members of the genus Brasilonema. It is concluded that heterocyte-forming cyanobacteria represent a component of the phyllosphere that is still largely undersampled in subtropical/tropical forests.
URI: http://hdl.handle.net/10553/135410
ISSN: 2076-2607
DOI: 10.3390/microorganisms12122625
Source: Microorganisms [ISSN 2076-2607], v. 12, n. 12, 2625, (Diciembre 2024)
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