Please use this identifier to cite or link to this item:
http://hdl.handle.net/10553/134577
DC Field | Value | Language |
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dc.contributor.author | Romero Alemán, María Del Mar | en_US |
dc.contributor.author | Pérez Galván, José Manuel | en_US |
dc.contributor.author | Hernández Rodríguez, José Enrique | en_US |
dc.contributor.author | Monzón Mayor,Maximina | en_US |
dc.date.accessioned | 2024-10-29T18:34:08Z | - |
dc.date.available | 2024-10-29T18:34:08Z | - |
dc.date.issued | 2024 | en_US |
dc.identifier.issn | 1549-3296 | en_US |
dc.identifier.uri | https://onlinelibrary.wiley.com/doi/10.1002/jbm.a.37825 | - |
dc.identifier.uri | http://hdl.handle.net/10553/134577 | - |
dc.description.abstract | This pilot study investigated the potential of aloe vera (AV) to promote neurite outgrowth in organotypic dorsal root ganglia (DRG) explants (n = 230) from neonatal rats (n = 15). Using this in vitro model of acute axotomy, we assessed neurite outgrowth exceeding 1.5 times the explant diameter (viable explants) and measured the longest neurite length. These parameters were evaluated under control conditions and in cultures supplemented with commercial AV and four aligned scaffolds: poly-L-lactate (PLLA), polydioxanone (PDS), poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), and blended PHBV/AV. After 6 days of culture, explants were immunostained using neuron-specific marker Tuj1 and Schwann cell marker S100. Measurements were obtained with Image J software and analyzed using Jamovi 2.3. In control and AV dilution media, the study revealed radial tissue growth from the explant body with randomly oriented neurites, whereas in all scaffolds, bidirectional tissue growth occurred parallel to nanofibers. Binomial logistic regression analyses indicated that viable explants were more likely in the control group compared to PDS (p = 0.0042) and PHBV (p < 0.0001), with non-significant differences when compared to AV dilution, PLLA, and PHBV/AV. AV dilution showed a greater association with viable explants than PLLA (p = 0.0459), while non-significant difference was found between AV dilution and PHBV/AV. Additionally, the PHBV/AV scaffold predicted higher odds of viable explants than PLLA (p = 0.0479), PDS (p = 0.0001), and PHBV (p < 0.0001). Groups with similar probabilities of obtaining viable explants (control, AV dilution, and PHBV/AV) exhibited non-significant differences in their longest neurite lengths. In conclusion, control, AV dilution, and PHBV/AV yielded the highest probability of developing viable explants and the longest neurite lengths. This is the first study demonstrating the direct permissiveness of AV for axonal outgrowth. Furthermore, the blended PHBV/AV scaffold showed significant potential as a suitable scaffold for axonal regrowth and Schwann cell migration, ensuring controlled tissue formation for tissue engineering applications. | en_US |
dc.language | eng | en_US |
dc.relation | Polímeros biológicos (Aloe Vera) y sintéticos (pds,plla) como soportes de le regeneración axonal en el ganglio de ....(PIA) | en_US |
dc.relation | Nanopolímeros sintéticos y naturales (Aloe Vera y miel) en el proceso de reinervación de la piel de ratón | en_US |
dc.relation | Utilidad de Productos Naturales (Aloe Vera, Miel)y Sus Derivados Biotécnicos (Nanopolímeros) en la Regeneración de la Vía Óptica (Snc) Del Reptil Canario G. Gallotiy de Las Heridas Cutáneas (Snp) en Modelos Animales (Murioy Porcino) Extrapolables A Humanos. | en_US |
dc.relation | “Nanopolímeros sintéticos y naturales en el proceso de reinervación de heridas escisionales cutáneas en ratones: Modelo experimental de cicatrización humana por segunda intención” | en_US |
dc.relation | Proyecto de Investigación "Nanopolímeros Sintéticos y Naturales en el Proceso de Reinervación de Heridas Escisionales Cutáneas en Ratos, Modelo Experimental de Cicatrización Humana Por Segunda Intención. Parte Ii: Análisis Estadístico de Resultados y Tramitación de Patentes" | en_US |
dc.relation | Actividad escolar con la ULPGC para la Impartición de diversas charlas orientadas a aplicaciones de productos elaborados de manera artesanal como la miel y aloe, de carácter divulgativo, dándoles un valor añadido a través de la Investigación como generadora de conocimientos aplicados a las Ciencias de la Salud, a los estudiantes de cuarto curso de la ESO, Bachiller y Ciclos Formativos | en_US |
dc.relation.ispartof | Journal of Biomedical Materials Research - Part A | en_US |
dc.source | Journal of Biomedical Materials Research - Part A [ISSN 1549-3296], (Octubre 2024) | en_US |
dc.subject | 32 Ciencias médicas | en_US |
dc.subject | 3201 Ciencias clínicas | en_US |
dc.subject.other | Aligned scaffolds | en_US |
dc.subject.other | Axon regeneration | en_US |
dc.subject.other | Electrospinning | en_US |
dc.subject.other | Organotypic DRG explants | en_US |
dc.subject.other | Processed aloe vera | en_US |
dc.subject.other | Tissue engineering | en_US |
dc.title | The Potential of Aloe Vera in Solution and in Blended Nanofibers Containing Poly (3‐Hydroxybutyrate‐Co‐3‐Hydroxyvalerate) as Substrates for Neurite Outgrowth | en_US |
dc.type | info:eu-repo/semantics/article | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.1002/jbm.a.37825 | en_US |
dc.investigacion | Ciencias de la Salud | en_US |
dc.type2 | Artículo | en_US |
dc.description.notas | Proyecto: Mancomunidad del Sureste de Gran Canaria (C2017-13). | en_US |
dc.description.numberofpages | 16 | en_US |
dc.utils.revision | Sí | en_US |
dc.date.coverdate | Octubre 2024 | en_US |
dc.identifier.ulpgc | Sí | en_US |
dc.contributor.buulpgc | BU-MED | en_US |
dc.description.sjr | 0,807 | |
dc.description.jcr | 4,9 | |
dc.description.sjrq | Q1 | |
dc.description.jcrq | Q2 | |
dc.description.scie | SCIE | |
dc.description.miaricds | 11,0 | |
item.fulltext | Con texto completo | - |
item.grantfulltext | open | - |
crisitem.author.dept | GIR IUIBS: Farmacología Molecular y Traslacional | - |
crisitem.author.dept | IU de Investigaciones Biomédicas y Sanitarias | - |
crisitem.author.dept | Departamento de Morfología | - |
crisitem.author.dept | Departamento de Morfología | - |
crisitem.author.dept | Departamento de Enfermería | - |
crisitem.author.dept | GIR IUIBS: Tecnología Médica y Audiovisual | - |
crisitem.author.dept | IU de Investigaciones Biomédicas y Sanitarias | - |
crisitem.author.orcid | 0000-0002-7987-5509 | - |
crisitem.author.orcid | 0000-0002-5437-1902 | - |
crisitem.author.orcid | 0000-0002-5046-508X | - |
crisitem.author.parentorg | IU de Investigaciones Biomédicas y Sanitarias | - |
crisitem.author.parentorg | IU de Investigaciones Biomédicas y Sanitarias | - |
crisitem.author.fullName | Romero Alemán, María Del Mar | - |
crisitem.author.fullName | Pérez Galván, José Manuel | - |
crisitem.author.fullName | Hernández Rodríguez, José Enrique | - |
crisitem.author.fullName | Monzón Mayor,Maximina | - |
crisitem.project.principalinvestigator | Monzón Mayor,Maximina | - |
crisitem.project.principalinvestigator | Hernández Rodríguez, José Enrique | - |
crisitem.project.principalinvestigator | Romero Alemán, María Del Mar | - |
crisitem.project.principalinvestigator | Romero Alemán, María Del Mar | - |
crisitem.project.principalinvestigator | Romero Alemán, María Del Mar | - |
crisitem.project.principalinvestigator | Hernández Rodríguez, José Enrique | - |
crisitem.project.principalinvestigator | Monzón Mayor,Maximina | - |
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