https://accedacris.ulpgc.es/jspui/handle/10553/30004 2026-04-15T21:08:56Z https://accedacris.ulpgc.es/jspui/handle/10553/156931 Título: Wnt/β-catenin signalling assists cell fate decision making in the early mouse embryo Autores/as: Lilao Garzón,Joaquín; Corujo Simon, Elena; Vinyoles, Meritxell; Fischer, Sabine C.; Guillén Salgado, José Ángel; Balayo, Tina; Muñoz Descalzo, Silvia Resumen: Cell fate choice is a key event happening during preimplantation mouse development. From embryonic day 3.5 (E3.5) to E4.5, the inner cell mass (ICM) differentiates into epiblast (Epi, NANOG expressing cells) and primitive endoderm (PrE, GATA6, SOX17 and/or GATA4 expressing cells). The mechanism by which ICM cells differentiate into Epi cells and PrE cells remains partially unknown. FGF/ERK has been proposed as the main signalling pathway for this event, but it does not explain co-expression of NANOG and GAT6 or how the cell fate choice is initiated. In this study, we investigate whether Wnt/β-catenin signalling also plays a role. To this end, we use two in vitro models based on inducible GATA6 expression: one in 2D, and another in 3D, namely ICM organoids. By combining these in vitro models with in vivo mouse embryos, chemical and classical genetics, and quantitative 3D immunofluorescence analyses, we propose a dual role for Wnt/β-catenin signalling. We find that β-catenin, acting alongside FGF/ERK signalling, helps to guide the cell fate choice towards PrE. Additionally, by regulating GATA6 and GATA4 stability, β-catenin further facilitates this choice. To summarise, we observe that pathway activation promotes PrE differentiation, while its inhibition stalls it. 2025-01-01T00:00:00Z https://accedacris.ulpgc.es/jspui/handle/10553/156929 Título: Regulation of mouse blastocyst primitive endoderm differentiation by p38-mitogen-activated-kinases (p38-MAPKs) is inner-cell-mass (ICM) autonomous and unrelated to cavity expansion defects in ICM specification Autores/as: Bohuslavová (née Stiborová), Martina; Hauserová, Andrea; Collier, Rebecca; Lilao Garzón,Joaquín; Muñoz Descalzo, Silvia; Bruce, Alexander W. Resumen: During early mouse blastocyst ICM maturation, we previously described that pharmacological inhibition of p38-MAPK (p38-MAPKi) significantly impairs primitive endoderm (PrE) differentiation from an initially uncommitted population of ICM cells but does not affect pluripotent epiblast (EPI) specification. A recent report details a positive role for blastocyst cavity expansion in assisting ICM lineage formation and marker gene expression. As p38-MAPKi also results in smaller cavity volumes, we addressed to what extent p38-MAPKi mediated impaired PrE differentiation is driven by ICM autonomous or cavity expansion mechanisms. We compared ICM differentiation phenotypes associated with either chemically inhibited cavity volume expansion and p38-MAPKi, on the individual cell and ICM lineage population levels. Whilst recapitulating previously observed decreases in expression of both EPI and PrE markers, we discovered cavity expansion phenotypes are manifest in impaired numbers of specified EPI and increased numbers of uncommitted cells; rather than impaired PrE differentiation, as observed after p38-MAPKi. Moreover, using both 2D ES-cell and 3D ICM organoid models, we show PrE differentiation is also significantly impaired by p38-MAPKi in the absence of a blastocyst cavity; a result recapitulated in cultured immuno-surgically isolated early blastocyst ICMs, in which an outer PrE and inner EPI population are ordinarily formed. These data confirm the early blastocyst requirement for p38-MAPK activity to permit PrE differentiation from uncommitted ICM progenitors is primarily ICM autonomous rather than caused by impaired cavity expansion. 2025-01-01T00:00:00Z https://accedacris.ulpgc.es/jspui/handle/10553/155784 Título: Consideraciones sobre la intensificación pragmática: incidencia de la imagen y propuesta de funciones Autores/as: Cedrés Pérez, Carlos Resumen: Este artículo parte de los principales estudios que versan sobre la intensificación pragmática, y contiene dos partes interrelacionadas. Por un lado, se reflexiona sobre un posible criterio de identificación del fenómeno de la intensificación: la incidencia de la imagen. Se pretende así contribuir a delimitar esta categoría, para facilitar su reconocimiento en los estudios de corpus. La metodología empleada ha sido inductivo-deductiva, partiendo de los corpus PRESEEA-Las Palmas y Ameresco-Las Palmas. Para la ejemplificación y reflexión se ha partido de ejemplos reales de dichos corpus. Por otro lado, se propone, como resultado de la reflexión anterior, una nueva taxonomía de funciones de la intensificación que permita la descripción pormenorizada de los actos de habla intensificados pragmáticamente en futuras investigaciones sociolingüísticas; This article is based on the main studies on pragmatic intensification and contains two interrelated parts. On the one hand, it reflects on a possible indicator for identifying intensification: namely the incidence of the face. It is thus intended to contribute to delimit this category, to facilitate its recognition in the corpus study. The methodology used was inductive-deductive, based on the PRESEEA-Las Palmas and Ameresco-Las Palmas corpora. Real examples from these corpora were used for illustration and reflection. On the other hand, as a result of the aforementioned reflection, a new taxonomy of intensification functions is proposed that allows for a detailed description of pragmatically intensified speech acts in future sociolinguistic research 2025-01-01T00:00:00Z https://accedacris.ulpgc.es/jspui/handle/10553/149561 Título: Description and Analysis of the Influence of Social Support on the Postoperative Evolution of Adult Patients Undergoing Retinal Detachment Surgery Autores/as: Castellano Santana, Pedro Raúl; Cabrera López, Francisco Antonio; Martín-Alonso, María De Las Nieves; Flores Jardo, Yésica; González-Martín, Jesús María; Díaz-Ginory, Ariday Miguel; Torres-Duchement, Abián-David; Santana Socorro, Yurena Del Carmen; Hernández Rodríguez, José Enrique Resumen: Background and Objectives: retinal detachment is an ophthalmological emergency requiring immediate surgical intervention and comprehensive management. This study evaluated the in-fluence of Perceived Social Support (PSS) on postoperative outcomes, including reintervention rates, adherence to recommendations, pain management, and local complications. It was hy-pothesized that higher levels of PSS would be associated with improved postoperative outcomes. Materials and Methods: A prospective study was conducted involving 166 patients who under-went retinal detachment surgery at a tertiary hospital (2022–2024). Sociodemographic and clinical data were collected, and PSS was assessed using the Medical Outcomes Study (MOS) question-naire, which demonstrated a Cronbach’s alpha reliability coefficient of 0.96. Dependent variables included reintervention rates, adherence, analgesic consumption, and local complications. De-scriptive and inferential statistical analyses were performed, with significance set at p < 0.05. Results: PSS scores were high (median: 5; SD: 0.55). Adherence rates were excellent (100% at 7 and 15 days; 99.04% at 30 days). Reintervention rates were low (0.5%, 1.0%, and 1.5% at 7, 15, and 30 days, respectively). The need for additional analgesia significantly decreased (46.43% at 7 days versus 13.33% at 30 days; SD: 6.0 to 3.2). No significant associations were found between PSS and major clinical variables, although higher levels of preoperative anxiety were associated with greater analgesic use (p = 0.041). Conclusions: PSS positively influences adherence and emotional well-being, highlighting its relevance in comprehensive postoperative management. These findings support the integration of tailored psychosocial and educational strategies alongside surgical treatment. 2024-01-01T00:00:00Z